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T7539 Sigma-Aldrich

Tumor Necrosis Factor-α from mouse

TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

Synonym: TNF-α, mTNF-α

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Properties

Related Categories Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines & Growth Factors,
biological source   mouse
recombinant   expressed in E. coli
assay   ≥95%
form   powder
potency   0.01-0.5 ng/mL EC50
mol wt   17 kDa
packaging   pkg of 10 μg
  pkg of 50 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   ≤1 EU/μg protein endotoxin
color   white
solubility   water: soluble
suitability   suitable for cell culture
UniProt accession no.   P06804
storage temp.   −20°C
Gene Information   mouse ... Tnf(21926)

Description

Application

Tumor necrosis factor-α (TNF-α) is a potent proinflammatory cytokine that plays a central role in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication, and hematopoiesis.

Biochem/physiol Actions

Tumor necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is glycosylated.

Unit Definition

One unit is the amount required to induce half-maximal cytolysis of L929 cells.

Physical form

Lyophilized from 10 mM sodium phosphate, pH 7.5

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Cell Culture Guide
Protocols & Articles

Articles

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Biofiles v6 n4, 9
Keywords: Angiogenesis, Antivirals, Apoptosis, Biological processes, Cardiovascular, Catalog, Catalysis, Cell culture, Cell proliferation, Deposition, Diabetes, Events, Gene expression, Growth factors, Hormones, Inflammation, Metabolism, Metabolites, Obesity, Phosphorylations, Physiological Processes, Reductions, Transcription, Transduction, Type

Tumor Necrosis Factor (TNF) Superfamily

The tumor necrosis factor (TNF) superfamily comprise an increasing number of structurally related ligand-receptor pairs, including TNF-α, TNF-β (lymphotoxin, LT), TNFR1, TNFR2, LT-βR, osteoprotegrin ...
Jennifer Fries
BioFiles 2009, 4.5, 23.
Keywords: Apoptosis, Biofiles, Cell disruption, Ligands, Microscopy, Type

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Peer-Reviewed Papers
15

References

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