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T7941 Sigma-Aldrich

Monoclonal Anti-β-Tubulin IV antibody produced in mouse

clone ONS.1A6, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
conjugate   unconjugated
clone   ONS.1A6, monoclonal
biological source   mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 1:400 using cultured human breast adenocarcinoma cell line MCF-7
species reactivity   rat, human, hamster, chicken, mouse, bovine
mol wt   antigen ~55 kDa
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG1
antibody product type   primary antibodies
Gene Information   human ... TUBB4(10382)
mouse ... Tubb4(22153)
rat ... Tubb4(29213)

Description

General description

Microtubules are heterodimeric proteins that comprise of α- and β-tubulins. β-tubulins have four different isotypes in brain cells, ranging from β I to β IV . Anti-tubulin antibodies are useful to study the localization of tubulin proteins. Monoclonal Anti-β-Tubulin IV antibody is specific for epitope located in the C-terminal sequence of β-tubulin isotype IV in humans, bovines, hamsters, rats, mice and chickens. The product does not react with other tubulin isotypes.

β-Tubulin IV codes for β-tubulin 4A (TUBB4A). TUBB4A is expressed in the central nervous system by oligodendrocytes. It is located on human chromosome 19p13.3.

Immunogen

synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype IV, conjugated to BSA.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunofluorescence (1 paper)
Western Blotting (1 paper)

Monoclonal Anti-β-Tubulin IV antibody has been used in immunostaining.

Monoclonal Anti-β-Tubulin IV antibody is suitable for use in immunohistochemistry (formalin-fixed, paraffin-embedded sections), indirect ELISA, microarray and western blot (1:400 using cultured human breast adenocarcinoma cell line MCF-7). The product may also be used for immunocytochemistry and immunofluorescence .

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

β-Tubulin IV creates the oligodendrocyte microtubule network, that helps in the transport of myelin proteins, lipids and RNA at the time of myelination. It participates in the generation of early fibrotic stages. TUBB4 plays a major role in the transport and proper localization of N-cadherin within the plasma membrane.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of dist...
Keywords: Enzyme-linked immunosorbent assay, Gene expression, Western blot

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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