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T8158 Sigma-Aldrich

Transferrin human

powder, BioReagent, suitable for cell culture

Synonym: Siderophilin, partially saturated

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Properties

Related Categories Albumins and Transport Proteins, Cell Culture, Hybridoma Media Supplements, Hybridoma Platform, Reagents and Supplements,
Quality Level   200
product line   BioReagent
assay   ≥98%
form   powder
concentration   ~25 mM
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
  hepatitis and HIV antibody, none detected
solubility   H2O: 50 mg/mL
shipped in   ambient
storage temp.   −20°C
Gene Information   human ... TF(7018)

Description

General description

Transferrin is a glycoprotein with a branched carbohydrate chain, having two sites for ferric ion binding in its polypeptide chain.

Application

Transferrin human has been used:
• to incubate human proximal tubule cells (HKC) - 8 cells along with IgG for the examination of albumin specificity
• as a supplement in serum-containing/devoid medium for human thyroid cell culture
• as a target glycoprotein to develop an in-gel hydrazinolysis method

Biochem/physiol Actions

Transferrin mediates iron transportation from plasma to cells. The iron-bound transferrin are of three forms: monoferric A/B transferrin and diferric transferrin. The transferrin binds to specific cell surface receptors and are internalized by endocytosis. Once inside, the iron is released into the cells and the iron-free transferrin, also referred to as apotransferrin, is exocytosed outside the cell without being degraded. Transferrin is known to deliver iron to cells in a physiologically safe and effective form.

Other Notes

Partially iron-saturated

Preparation Note

Heat treated at 60 °C for ten hours.

Analysis Note

Purity by agarose electrophoresis.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the carbohydrate content of human transferrin?
We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.
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Protocols & Articles

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Peer-Reviewed Papers
15

References

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