• USA Home
  • T8300 - Anti-Tumor Necrosis Factor-α antibody produced in rabbit

T8300 Sigma-Aldrich

Anti-Tumor Necrosis Factor-α antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-TNF-α



Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   dot blot: 1:2,000
  microarray: suitable
  neutralization: 1-3 μg/mL
species reactivity   human
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P01375
Gene Information   human ... TNF(7124)


General description

Tumor necrosis factor- α(TNF-α), also known as cachectin, is an inflammatory cytokine that exists primarily as a 51kDa complex built up of 3 identical, non-covalently-linked polypeptide subunits (17 kDa, 157 amino acid residues, in human). TNF- αoccurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. TNF-α is secreted by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes, endothelial cells, smooth muscle cells, and some transformed cells.


The antibody binds to TNF-α. It does not cross-react with recombinant human TNF-β, IL-1a, IL-1β or IL-3, nor with recombinant mouse or human IL-6.


recombinant human TNF-α produced in bacteria.


Anti-Tumor Necrosis Factor-α antibody may be used for dot blot at a working antibody dilution of 1:2000. The antibody may be used for neutralization assay; the ND50 is 1-3 μg/ml. Anti-TNF-α antibody was used for neutralization of TNF-α of Jurkat cells.

Anti-Tumor Necrosis Factor-α antibody produced in rabbit has been used in western blotting. It may also be used in enzyme linked immunoassay (ELISA).


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Tumor Necrosis Factor-α (TNF-α) is a cytokine produced by macrophages in response to stimulus by LPS. It plays a major role in mediating inflammation, tissue injury, pathogenic shock, innate immunity, apoptosis and autoimmunity. The physiological effects of TNF-α are mediated by receptors of TNFR super family. The TNF/TNFR signaling axis plays a critical role in mediating either survival or apoptosis depending on the class of adaptor proteins recruited in the cell. Association of receptors with a death domain causes rapid activation of caspases and results in cell death. TNF/TNFR also results in the activation of downstream pathways involving MAPK, p38, JNK and NF-κB that play a major role in innate immunity, acute inflammatory responses and homeostasis. The TNF/TNFR axis is also important in the pathology of various autoimmune and inflammatory disorders including a variety of malignancies and Alzheimer′s disease.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?