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T8575 Sigma-Aldrich

Anti-Tryptophan Hydroxylase antibody produced in sheep

affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-TPH

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Neurotransmission, Antibodies for Neurotransmitter Synthesis and Metabolism,
conjugate   unconjugated
clone   polyclonal
biological source   sheep
application(s)   western blot: 1:1,000 using human dorsal raphe nucleus
species reactivity   human, mammals
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
usage   sufficient for 10 blots
UniProt accession no.   P17752
Gene Information   human ... TPH1(7166)
mouse ... Tph1(21990)
rat ... Tph1(24848)

Description

General description

The tryptophan hydroxylase 1 (TPH1) gene spanning 29kbp with 11 exons is mapped to human chromosome 11p15.3-p14. TPH1, is a 444 amino acid protein expressed in the gut, spleen, thymus and also in the pineal gland and the pituitary.

Immunogen

recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunohistochemistry (1 paper)

Physical form

Solution in 150 mM NaCl, 10 mM HEPES, pH 7.5, 100 μg per mL BSA, and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The tryptophan hydroxylase 1 gene (TPH1) catalyzes the synthesis of 5-hydroxytryptophan, which is a precursor to the neurotransmitter serotonin. Variation in the gene expression affects prefrontal cortex activation during response inhibition. Mutation in the TPH1 gene leads to schizophrenia, irritable bowel syndrome (IBS) in women, suicidal behavior and depressive disorders. The encoded protein plays a vital role in regulation of cardiovascular function.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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