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T8778 Sigma-Aldrich

Trehalase from porcine kidney

buffered aqueous glycerol solution, ≥1.0 units/mg protein

Synonym: α,α-Trehalose glucohydrolase



Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
assay   0.5-10.0 mg/mL protein basis (BCA)
form   buffered aqueous glycerol solution
storage temp.   −20°C


General description

Trehalose is the main haemolymph sugar in most insects and is potentially a prime target for an invading pathogenic fungus.


Trehalase has been used in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves. Trehalase has also been used in a study to investigate growth arrest by trehalose-6-phosphate.

Unit Definition

One unit will convert 1.0 μmole of trehalose to 2.0 μmoles of glucose per min at pH 5.7 at 37 °C (liberated glucose determined at pH 7.5).

Physical form

Solution in 50% glycerol containing 1% Triton X-100 and 25 mM potassium phosphate, pH 6.5

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
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The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How is Product T8778, Trehalase from porcine kidney prepared?
The actual method of preparation utilized by Sigma for Product T8778, Trehalase is confidential, but does include ion exchange and size exclusion chromatography. A method of preparation for Trehalase has been described in Archives of Biochemistry and Biophysics, 255, 168 - 175 (1987).
What is the molecular weight of Product T8778, Trehalase from porcine kidney?
The molecular weight of Product T8778, Trehalase from porcine kidney has an estimated molecular weight of 80,000 daltons upon SDS-PAGE, as described byYoshitaka Yoneyama in Archives of Biochemistry and Biophysics, 255, 168 - 175 (1987). This molecular weight is similar to that reported for mouse Trehalase which has an estimated molecular weight by gel filtration on Sephadex G-200 of 73,000 daltons, as described in Anais Da Academia Brasileira De Ciencias, 51, 151 - 158 (1979).
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