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T9281 Sigma

N,N,N′,N′-Tetramethylethylenediamine

BioReagent, suitable for electrophoresis, ~99%

Synonym: 1,2-Bis(dimethylamino)ethane, TEMED, TMEDA

  • CAS Number 110-18-9

  • Linear Formula (CH3)2NCH2CH2N(CH3)2

  • Molecular Weight 116.20

  •  Beilstein Registry Number 1732991

  •  EC Number 203-744-6

  •  MDL number MFCD00008335

  •  PubChem Substance ID 24900591

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Description

Analysis Note

Tested as a catalyst for PAGE.

Caution

Handle and store under inert gas, air and moisture sensitive.

Packaging

25, 50, 100 mL in glass bottle

Application

TEMED (N,N,N′,N′-Tetramethylethylenediamine) has been used for the preparation of acrylamide hydrogels and sodium dodecyl sulfate-polyacrylamide gels.

General description

TEMED (N,N,N′,N′-Tetramethylethylenediamine) is molecule which allows rapid polymerization of polyacrylamide gels. In the presence of ammonium persulfate, TEMED is responsible for the formation of free radicals from persulfate, thereby initiating the acrylamide polymerization process. However, excess concentration of TEMED can result in abnormal separation patterns.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2372 3 / PGII
WGK Germany 
1
RTECS 
KV7175000
Flash Point(F) 
68 °F
Flash Point(C) 
20 °C

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Protocols & Articles

Articles

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

NMR Analysis of Glycans

Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field. This technique is non-destructive and can provide full structural info...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Cancer, Digestions, Electrophoresis, Nuclear magnetic resonance spectroscopy, Separation, Spectroscopy

Protocols

Design and assessment of engineered CRISPR–Cpf1 and its use for genome editing

a Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University
Keywords: Cancer, Clinical, Oncology, Pharmaceutical, Sequencing

Tricine–SDS-PAGE

a Molekulare Bioenergetik, Zentrum der Biologischen Chemie, Universitätsklinikum Frankfurt, Theodor-Stern-Kai 7, Haus 26
Keywords: Buffers, Chromatography, Crystallization, Degradations, Detergents, Edman degradation, Electroblotting, Electrophoresis, Gel electrophoresis, PAGE, Phosphorylations, Polymerization reactions, Proteomics, Purification, Respiratory, SDS PAGE, Sample preparations, Separation, Sequencing, Solvents, Western blot

Peer-Reviewed Papers
15

References

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