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T9281 Sigma-Aldrich

N,N,N′,N′-Tetramethylethylenediamine

BioReagent, suitable for electrophoresis, ~99%

Synonym: 1,2-Bis(dimethylamino)ethane, TEMED, TMEDA

  • CAS Number 110-18-9

  • Linear Formula (CH3)2NCH2CH2N(CH3)2

  • Molecular Weight 116.20

  •  Beilstein/REAXYS Number 1732991

  •  EC Number 203-744-6

  •  MDL number MFCD00008335

  •  PubChem Substance ID 24900591

  •  NACRES NB.22

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Properties

Related Categories Core Bioreagents, Electrophoresis of Glycans, Gel Casting Reagents, General Reagents, Glycan Labeling and Analysis,
Quality Level   200
product line   BioReagent
assay   ~99%
expl. lim.   9.08 %
refractive index   n20/D 1.4179 (lit.)
bp   120-122 °C (lit.)
mp   −55 °C (lit.)
density   0.775 g/mL at 20 °C (lit.)
suitability   suitable for electrophoresis
SMILES string   CN(C)CCN(C)C
InChI   1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3
InChI key   KWYHDKDOAIKMQN-UHFFFAOYSA-N

Description

General description

TEMED (N,N,N′,N′-Tetramethylethylenediamine) is molecule which allows rapid polymerization of polyacrylamide gels. In the presence of ammonium persulfate, TEMED is responsible for the formation of free radicals from persulfate, thereby initiating the acrylamide polymerization process. However, excess concentration of TEMED can result in abnormal separation patterns.

Application

TEMED (N,N,N′,N′-Tetramethylethylenediamine) has been used for the preparation of acrylamide hydrogels and sodium dodecyl sulfate-polyacrylamide gels.

Packaging

25, 50, 100 mL in glass bottle

Caution

Handle and store under inert gas, air and moisture sensitive.

Analysis Note

Tested as a catalyst for PAGE.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2372 3 / PGII
WGK Germany 
WGK 1
RTECS 
KV7175000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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pHast Pack
Protocols & Articles

Articles

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

NMR Analysis of Glycans

Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field. This technique is non-destructive and can provide full structural info...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Cancer, Digestions, Electrophoresis, Nuclear magnetic resonance spectroscopy, Separation, Spectroscopy

Peer-Reviewed Papers
15

References

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