• USA Home
  • T9283 - Monoclonal Anti-Tropomyosin (Sarcomeric) antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
T9283 Sigma-Aldrich

Monoclonal Anti-Tropomyosin (Sarcomeric) antibody produced in mouse

clone CH1, ascites fluid

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin,
conjugate   unconjugated
clone   CH1, monoclonal
biological source   mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using sections of human tongue
  immunohistochemistry (frozen sections): suitable using human and animal tissue sections
  immunoprecipitation (IP): suitable
  microarray: suitable
  western blot: suitable using native preparations
species reactivity   chicken, human, rat
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG1
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P09493
Gene Information   chicken ... TPM1(396366)
human ... TPM1(7168)
rat ... Tpm1(24851)

Description

General description

Tropomyosin 1 (TPM1) is encoded by the gene mapped to human chromosome 15q22.2. The encoded protein belongs to the TPM protein family.

Specificity

The antibody recognizes an epitope on skeletal and cardiac tropomyosin. The antibody shows no reaction with denatured protein by immunoblotting. No cross-reactivity has been observed with smooth muscle or non-muscle tropomyosin.

Immunogen

chicken muscle tropomyosin.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Monoclonal Anti-Tropomyosin (sarcomeric) antibody produced in mouse has been used in immunofluorescence and immunohistochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Tropomyosin 1 (TPM1) is a potent tumor suppressor gene. It is expressed at low levels in various types of solid cancers including, oral squamous cell carcinoma (OSCC) and tongue squamous cell carcinoma. Mutation in the gene is associated with the development of hypertrophic cardiomyopathy. The encoded protein is involved in the regulation of actin-myosin interaction in striated muscles. Tropomyosin plays an essential role in Ca2+ -regulated contraction of cardiac muscle.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?