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T9285 Sigma-Aldrich

Tris-EDTA buffer solution

100X concentrate for Northern and Southern blotting

Synonym: TE buffer solution

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts, Buffer Convenience Packaging, Buffer Solutions,
grade   for molecular biology
InChI Key   VLEIUWBSEKKKFX-UHFFFAOYSA-N
sterility   0.2 μm filtered
foreign activity   DNase, RNase, none detected
storage temp.   room temp

Description

Components

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Application

Suitable for storage and isolation of RNA and DNA, including purified plasmid DNA stocks.
• Tris-EDTA buffer solution has been used in culture medium.
• Tris-EDTA buffer solution has been used for the preparation of AmpC disks for permeabilization of Tris-EDTA to release β-lactamases from bacterial cell.
• It has been used for the suspension of bacterial spores.
• It has also been used as a buffer in immunohistochemistry.

General description

TE buffer is the most commonly used buffer solution in molecular biology. "TE" is a component of Tris (a common pH buffer) and EDTA (molecule that chelate cations). TE (Tris EDTA) Buffer solubilizes DNA and RNA while protecting it from degradation. TE Buffer is used in nucleic acid isolation, which may be done prior to Northern or Southern blot hybridization.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What are the final concentrations of Tris and EDTA when Product T9285 is diluted to 1:100 to make it 1X?
When diluted 1:100, the solution is 10mM TRIS with 1mM EDTA.
What is the pH and color of Product T9285, Tris-EDTA buffer solution?
The pH and color information is available on the specification sheet as well as lot specific Certificate of Analysis.
What is the shelf life of Product T9285, Tris-EDTA buffer solution?
This buffer solution has a recommended retest date of three years. This information is available on the Specification Sheet, and on the lot-specific Certificates of Analysis.
What does 'for molecular biology' mean?
The phrase `for molecular biology' means that all lots of this buffer have been tested for DNase and RNase activity, and that no activity was detected.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Peer-Reviewed Papers
15

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