• USA Home
  • T9378 - Trypsin inhibitor from Phaseolus limensis (lima bean)

EMAIL THIS PAGE TO A FRIEND
T9378 Sigma-Aldrich

Trypsin inhibitor from Phaseolus limensis (lima bean)

Type II-L, crude powder

Synonym: Trypsin Inhibitor from lima beans

Purchase

Description

Application

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:
• in the inhibition of trypsin in human plasma
• in the termination of proteolytic digestion in rabbit skeletal muscles
• in the inhibition of salivary glands enzyme extract from Lygus lineolaris
• in the inhibition of proteolytic activity in Agave tequilana enzyme extract

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.

Packaging

1 g in glass bottle

100, 500 mg in glass bottle

Biochem/physiol Actions

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.

Physical properties

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

Unit Definition

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Preparation Note

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

Analysis Note

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Other Notes

View more information on Trypsin Inhibitor.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles

Articles

HPLC Analysis of Proteins on Discovery® BIO PolyMA-WAX (Analyte Set #1)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Discovery® BIO PolyMA-WAX (Analyte Set #1)
Keywords: Chromatography, High performance liquid chromatography, Separation

Protocols

Enzymatic Assay of Trypsin Inhibitor

3.2 Unit Definition – One Trypsin BAEE unit will produce a ΔA253nm of 0.001 per minute with BAEE as substrate at pH 7.6 at 25ºC in a reaction volume of 3.2 ml.

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?