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  • T9577 - Anti-p8/TTD-A (N-terminal) antibody produced in rabbit

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T9577 Sigma-Aldrich

Anti-p8/TTD-A (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-C6orf175 TFB5, Anti-Chromosome 6 open reading frame 175, Anti-GTF2H5, Anti-General transcription factor IIH, polypeptide 5, Anti-TFIIH basal transcription factor complex, TTDA subunit, Anti-TGF2H5, Anti-TTDA transcription factor IIH, 8-kd subunit

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling, Antibodies for Gene Regulation, Antibodies to Common Transcription Factors,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect immunofluorescence: 1:200-1:400 using paraformaldehyde-fixed HEK-293T cells, over-expressing human p8/TTD-A
  western blot: 1:250-1:500 using lysates of CHO or HEK-293T over-expressing human p8/TTD-A
species reactivity   human, mouse, rat
mol wt   antigen ~8 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
UniProt accession no.   Q6ZYL4
Gene Information   human ... GTF2H5(404672)
mouse ... Gtf2h5(66467)
rat ... Gtf2h5(502227)

Description

Target description

p8/TTD-A (N-terminal) encodes a subunit of transcription/repair factor TFIIH, which functions in gene transcription and DNA repair. This protein stimulates ERCC3/XPB ATPase activity to trigger DNA opening during DNA repair, and is implicated in regulating

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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T9702 Anti-p8/TTD-A (C-terminal) antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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