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TRACRRNA05N Sigma-Aldrich

SygRNA® Cas9 Synthetic tracrRNA

SygRNA® SpCas9 tracrRNA

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Properties

Related Categories CRISPR-Cas9, Functional Genomics and RNAi, Molecular Biology, Synthetic CRISPR
packaging   pkg of 5 nmol × Tube (HPLC)
shipped in   ambient
storage temp.   −20°C

Description

General description

The SygRNA® synthetic RNA system recruits SpCas9 nuclease to specific genomic targets. The tracrRNA (trans-activating crRNA) binds to a crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides Cas9 protein to crRNA-specified sites in the genome. SygRNA® synthetic tracrRNA and crRNAs are optimized for use with Cas9 expressing of cell lines, Cas9 protein, or Cas9 mRNA and compatible with a variety of delivery methods such as microinjection, lipofection, and electroporation.

Application

Functional Genomics/Transgenic Applications
• Creation of gene knockouts
• Creation of knock-in animals or cell lines with promoters, fusion tags or reporters integrated into endogenous genes

Features and Benefits

HPLC-purified SygRNA® Cas9 tracrRNA oligo is optimized for use with a target-specific cRNA and a source of SpCas9 protein for genome editing applications.

Linkage

The SygRNA® Cas9 tracrRNA oligo is used in conjunction with a target-specific crRNA and a source of Cas9 protein such as TGEN-CP-50UG, CAS9P, or CAS9MRNA to form active RNP complexes. Select predesigned unique crRNAs against human, mouse and rat genes at CRISPR search.

Learn more about SygRNA® tracrRNA

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Physical form

Dry

Preparation Note

Resuspend SygRNA® Cas9 tracrRNA oligo in a RNAase-free Tris-containing buffer with a pH 8.0 or less.

Legal Information

CRISPR Use License Agreement

SygRNA is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

SygRNA® Synthetic CRISPR guide RNAs

SygRNA® synthetic single guide RNAs (sgRNA), crRNAs and tracrRNAs are synthesized using our proprietary process and reagents using the highest quality materials currently available on the market. Edi...
Keywords: High performance liquid chromatography, Purification, Sequencing

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Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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