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TRUEA Sigma-Aldrich

TrueGel3D Buffer pH 5.5

600 μl solution, 10X concentrated



General description

TrueGel3D buffer pH 5.5 is a component of the TrueGel3D Hydrogel system. The TrueGel3D buffer with pH 5.5 is recommended for fast gelling hydrogels, because the acidic pH (lower pH) slows down the formation of a gel allowing homogenous mixing of polymer and crosslinker before it solidify. However, in conjugation with TrueGel3D buffer pH 7.2, buffer with intermediate strengths can be prepared to modulate gelation kinetics. Both buffer systems (TRUEA and TRUEB) have pH indicator (phenol red) and strong buffering capacity to ensure a stable pH.


TrueGel3D buffers with pH 5.5 and 7.2 is used to modulate gelation kinetics


TRUEA-1VL contains TrueGel3D buffer pH 5.5, 10X concentrated

Safety & Documentation

Safety Information

UN 1789 8 / PGIII


Certificate of Analysis

Protocols & Articles


True Gel3D™ Troubleshooting guide

From our library of Articles, Sigma-Aldrich presents True Gel3D™ Troubleshooting guide
Keywords: Adhesion

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a biochemically defined hydrogel formed by mixing polymers with crosslinkers. Compared to other hydrogels based on a biological extract from animal cells (e.g. mouse EHS tumor cells), T...
Keywords: Adhesion, Buffers, Cancer, Cell culture, Growth factors, Immobilization, Immunostaining, Ligands, Microscopy, PAGE, SDS PAGE


Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

The identity and concentration of peptides or proteins can be varied when constructing a TrueGel 3D™ hydrogel. This peptide concentration optimization may be important for specific applications such ...
Keywords: Adhesion, High performance liquid chromatography, Ligands, Oxidations

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