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TRUECD Sigma-Aldrich

TrueGel3D Crosslinker

CD cell-degradable crosslinker

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Description

General description

CD cell-degradable crosslinker is comprised of conjugated peptide along with polyethylene glycol and thiol (SH) groups. The thiol groups form stable thioether bond with thiol-reactive groups of the polymers (Mal-PVA, Mal-Dextran, SG-PVA and SG-Dextran) and results in a stable hydrogel.

The conjugated peptide has matrix metalloprotease (MMP)-cleavage sites (Pro-Leu-Gly-Leu-Trp-Ala), which allows matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9) to locally degrade the polymer network. It allows cells to spread and migrate within the gel if they express the indicated MMPs.

Application

TRUECD is used to crosslink PVA and dextran polymers to generate cell-degradable hydrogel

Features and Benefits

MMP degradable

Components

TRUECD contains CD cell-degradable crosslinker lyophilized and water.

Caution

Avoid prolong exposure of CD cell-degradable crosslinker to air

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles

Articles

True Gel3D™ Troubleshooting guide

From our library of Articles, Sigma-Aldrich presents True Gel3D™ Troubleshooting guide
Keywords: Adhesion

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a biochemically defined hydrogel formed by mixing polymers with crosslinkers. Compared to other hydrogels based on a biological extract from animal cells (e.g. mouse EHS tumor cells), T...
Keywords: Adhesion, Buffers, Cancer, Cell culture, Growth factors, Immobilization, Immunostaining, Ligands, Microscopy, PAGE, SDS PAGE

Protocols

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

The identity and concentration of peptides or proteins can be varied when constructing a TrueGel 3D™ hydrogel. This peptide concentration optimization may be important for specific applications such ...
Keywords: Adhesion, High performance liquid chromatography, Ligands, Oxidations

TrueGel3D™ Fast Gelling Hydrogels Preparation Protocol

TrueGel3D™ is a chemically-defined hydrogel formed by mixing polymers with crosslinkers. This hydrogel permits the encapsulation of cells to put them in a three-dimensional environment to better mimi...
Keywords: Adhesion, Buffers, Cell attachment, Cell culture, Crystallization, Oxidations

TrueGel3D™ Slow Gelling Hydrogels Preparation Protocol

TrueGel3D™ is a chemically-defined hydrogel formed by mixing polymers with crosslinkers. This hydrogel enables the encapsulation of cells to enable their growth in a 3-dimensional environment that be...
Keywords: Adhesion, Buffers, Cell attachment, Cell culture, Crystallization, Oxidations

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