EMAIL THIS PAGE TO A FRIEND
TRUEDEXF Sigma-Aldrich

TrueGel3D Polymer

Modified for fast gelling (FAST-DEXTRAN),allow cell recovery

Purchase

Description

General description

Dextrose polymers are functionalized with thiol-reactive maleimide groups, which react rapidly with thiol groups of crosslinkers to form hydrogels in few seconds. The thiol-reactive maleimide groups are also compatible to conjugate polymers with bioactive factors. The thioglycerol is used to maintain hydrogel strength and also as a control to compare cellular behavior in peptide-modified versus unmodified hydrogels. The buffers (pH7.2 and pH 5.5) are used to control pH and osmotic conditions to optimize time for gelation.

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

Application

FAST-DEXTRAN polymer is used with crosslinkers (CD/PEG crosslinkers) to establish stable hydrogels

Features and Benefits

• Contains dextrose polymer functionalized with maleimide groups for quick gelation
• Degradable polymer

Components

TRUEDEXF contains FAST-DEXTRAN lyophilized with reconstitution buffer, TrueGel3D thioglycerol and water

Caution

Avoid frequent freeze-thaw cycles and store FAST-DEXTRAN in small portions

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???
Protocols & Articles

Articles

True Gel3D™ Troubleshooting guide

From our library of Articles, Sigma-Aldrich presents True Gel3D™ Troubleshooting guide
Keywords: Adhesion

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a biochemically defined hydrogel formed by mixing polymers with crosslinkers. Compared to other hydrogels based on a biological extract from animal cells (e.g. mouse EHS tumor cells), T...
Keywords: Adhesion, Buffers, Cancer, Cell culture, Growth factors, Immobilization, Immunostaining, Ligands, Microscopy, PAGE, SDS PAGE

Protocols

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

The identity and concentration of peptides or proteins can be varied when constructing a TrueGel 3D™ hydrogel. This peptide concentration optimization may be important for specific applications such ...
Keywords: Adhesion, High performance liquid chromatography, Ligands, Oxidations

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?