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TRUEENZ Sigma-Aldrich

TrueGel3D Enzymatic Cell Recovery Solution

solution, 500 μl

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Description

Components

TRUEENZ-500UL contains TrueGel3D enzymatic cell recovery solution (dextranase)

Application

TrueGel3D enzymatic cell recovery solution is used to dissolve dextran-based hydrogels to recover cells for post-culture analysis

Caution

Avoid loss of enzymatic activity by storing at -70°C

General description

TrueGel3D enzymatic cell recovery solution is extracted from Chaetomium gracile, which cleave glycosidic bonds of dextran polymers. It is used to dissolve dextran polymer to recover chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

True Gel3D™ Troubleshooting guide

From our library of Articles, Sigma-Aldrich presents True Gel3D™ Troubleshooting guide
Keywords: Adhesion

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a biochemically defined hydrogel formed by mixing polymers with crosslinkers. Compared to other hydrogels based on a biological extract from animal cells (e.g. mouse EHS tumor cells), T...
Keywords: Adhesion, Buffers, Cancer, Cell culture, Growth factors, Immobilization, Immunostaining, Ligands, Microscopy, PAGE, SDS PAGE

Protocols

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

The identity and concentration of peptides or proteins can be varied when constructing a TrueGel 3D™ hydrogel. This peptide concentration optimization may be important for specific applications such ...
Keywords: Adhesion, High performance liquid chromatography, Ligands, Oxidations

TrueGel3D™ Fast Gelling Hydrogels Preparation Protocol

TrueGel3D™ is a chemically-defined hydrogel formed by mixing polymers with crosslinkers. This hydrogel permits the encapsulation of cells to put them in a three-dimensional environment to better mimi...
Keywords: Adhesion, Buffers, Cell attachment, Cell culture, Crystallization, Oxidations

TrueGel3D™ Slow Gelling Hydrogels Preparation Protocol

TrueGel3D™ is a chemically-defined hydrogel formed by mixing polymers with crosslinkers. This hydrogel enables the encapsulation of cells to enable their growth in a 3-dimensional environment that be...
Keywords: Adhesion, Buffers, Cell attachment, Cell culture, Crystallization, Oxidations

TrueGel3D™ hydrogel CD cell-degradable crosslinker and RGD peptide (TRUE1) preparation protocol

TrueGel3D™ is a chemically-defined hydrogel formed by mixing polymers with crosslinkers. This hydrogel enables the encapsulation of cells to permit their growth in a 3-dimensional environment to bett...
Keywords: Adhesion, Cell culture, Oxidations

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