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TSTOP Sigma

ThermaStop New

increases PCR specificity, inhibits non-specific polymerase activity, prevents mis-priming before PCR, improves hot start PCR

Synonym: Multiplex PCR, PCR Additive, hot start DNA polymerase, hot start PCR, hotstart

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Properties

assay   (Dual, HPLC)
form   lyophilized powder
packaging   pkg of One unit of ThermaStop is defined as the amount required for maximum hot-start in amplification reactions containing one unit of Taq DNA polymerase in a volume of 25 μl.
scale   500 and 2500 (units)
application(s)   PCR: suitable
color   opaque
shipped in   ambient
storage temp.   room temp

Description

Reconstitution

Dissolve in Tris pH 8.3 buffer

Application

• For PCR that requires reduced non-specific amplification, including for downstream applications with specific amplification products for NGS and Gibson assembly.
• For highly multiplex PCR
• For prevention of primer dimer formation

General description

ThermaStop is a reversible hot-start reagent that is compatible with thermostable DNA polymerases. It acts directly on the polymerase to prevent non-specific enzymatic activity below 50°C. Polymerase activity is fully restored at 60°C, but is inhibited again by ThermaStop by cooling the reaction. Adding ThermaStop to your PCR reactions improves sensitivity and total product yield.

Legal Information

ThermaStop is a trademark of ThermaGenix Inc.

Price and Availability

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles

Articles

ThermaGenix Product Overview

The polymerase chain reaction (PCR) is a mainstay of molecular biology and genomics that provides an efficient and rapid in vitro method for enzymatic amplification of DNA or RNA sequences from vario...
Keywords: Amplification, Diagnostic, Enzyme activity, Genomics, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Sequencing, Transcription

ThermaGo™ PCR Additive

The ThermaGo™ additive is a double-stranded, chemically modified nucleic acid that suppresses mis-priming during the annealing and extension steps of PCR. It increases the reproducibility of both rea...
Keywords: Amplification, Atomic absorption spectroscopy, Nucleic acid annealing, Phase transitions, Polymerase chain reaction, Separation, Sequencing

ThermaStop™ PCR Additive

Innovative Hot Start and Cold Stop Reagent for Enhanced Taq DNA Polymerase Specificity and Product Yield
Keywords: Amplification, Asymmetric synthesis, Enzyme activity, Nucleic acid annealing, Phase transitions, Polymerase chain reaction, Sequencing

ThermaStop™-RT PCR Additive

The ThermaStop™-RT additive is a simple-to-use reagent for one-step or two-step reverse transcription-PCR (RT-PCR) initiated with gene-specific primers. ThermaStop™-RT additive interacts with reverse...
Keywords: Amplification, Nucleic acid annealing, Phase transitions, Polymerase chain reaction, Transcription

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