ThermaStop New

increases PCR specificity, inhibits non-specific polymerase activity, prevents mis-priming before PCR, improves hot start PCR

Synonym: Multiplex PCR, PCR Additive, hot start DNA polymerase, hot start PCR, hotstart



assay   (Dual, HPLC)
form   lyophilized powder
packaging   pkg of One unit of ThermaStop is defined as the amount required for maximum hot-start in amplification reactions containing one unit of Taq DNA polymerase in a volume of 25 μl.
scale   500 and 2500 (units)
application(s)   PCR: suitable
color   opaque
shipped in   ambient
storage temp.   room temp



Dissolve in Tris pH 8.3 buffer


• For PCR that requires reduced non-specific amplification, including for downstream applications with specific amplification products for NGS and Gibson assembly.
• For highly multiplex PCR
• For prevention of primer dimer formation

General description

ThermaStop is a reversible hot-start reagent that is compatible with thermostable DNA polymerases. It acts directly on the polymerase to prevent non-specific enzymatic activity below 50°C. Polymerase activity is fully restored at 60°C, but is inhibited again by ThermaStop by cooling the reaction. Adding ThermaStop to your PCR reactions improves sensitivity and total product yield.

Legal Information

ThermaStop is a trademark of ThermaGenix Inc.

Price and Availability

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis

Protocols & Articles


ThermaGenix Product Overview

The polymerase chain reaction (PCR) is a mainstay of molecular biology and genomics that provides an efficient and rapid in vitro method for enzymatic amplification of DNA or RNA sequences from vario...
Keywords: Amplification, Diagnostic, Enzyme activity, Genomics, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Sequencing, Transcription

ThermaGo™ PCR Additive

The ThermaGo™ additive is a double-stranded, chemically modified nucleic acid that suppresses mis-priming during the annealing and extension steps of PCR. It increases the reproducibility of both rea...
Keywords: Amplification, Atomic absorption spectroscopy, Nucleic acid annealing, Phase transitions, Polymerase chain reaction, Separation, Sequencing

ThermaStop™ PCR Additive

Innovative Hot Start and Cold Stop Reagent for Enhanced Taq DNA Polymerase Specificity and Product Yield
Keywords: Amplification, Asymmetric synthesis, Enzyme activity, Nucleic acid annealing, Phase transitions, Polymerase chain reaction, Sequencing

ThermaStop™-RT PCR Additive

The ThermaStop™-RT additive is a simple-to-use reagent for one-step or two-step reverse transcription-PCR (RT-PCR) initiated with gene-specific primers. ThermaStop™-RT additive interacts with reverse...
Keywords: Amplification, Nucleic acid annealing, Phase transitions, Polymerase chain reaction, Transcription

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