TSTOP Sigma-Aldrich


increases PCR specificity, inhibits non-specific polymerase activity, prevents mis-priming before PCR, improves hot start PCR

Synonym: Multiplex PCR, PCR Additive, hot start DNA polymerase, hot start PCR, hotstart



Related Categories Molecular Biology, PCR Enhancers, PCR/Amplification More...
assay   (Dual, HPLC)
form   lyophilized powder
packaging   pkg of One unit of ThermaStop is defined as the amount required for maximum hot-start in amplification reactions containing one unit of Taq DNA polymerase in a volume of 25 μl.
scale   500 and 2500 (units)
application(s)   PCR: suitable
color   opaque
shipped in   ambient
storage temp.   2-8°C


General description

ThermaStop is a reversible hot-start reagent that is compatible with thermostable DNA polymerases. It acts directly on the polymerase to prevent non-specific enzymatic activity below 50°C. Polymerase activity is fully restored at 60°C, but is inhibited again by ThermaStop by cooling the reaction. Adding ThermaStop to your PCR reactions improves sensitivity and total product yield.


• For PCR that requires reduced non-specific amplification, including for downstream applications with specific amplification products for NGS and Gibson assembly.
• For highly multiplex PCR
• For prevention of primer dimer formation


Dissolve in Tris pH 8.3 buffer

Legal Information

ThermaStop is a trademark of ThermaGenix Inc.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


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