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U0251 Sigma-Aldrich

Urease from Canavalia ensiformis (Jack bean)

Type C-3, powder, ≥600,000 units/g solid

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Properties

Related Categories 3.5.x.x Acting on C-N other than peptides, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,
Quality Level   200
type   Type C-3
form   powder
specific activity   ≥600,000 units/g solid
mol wt   ~544620 Da
purified by   crystallization
storage temp.   −20°C

Description

General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620

Packaging

6000, 12000, 60000 units in glass bottle

Biochem/physiol Actions

Urease was shown to induce vacuolation in Hela cells, an effect dependent on the ability of urease to convert urea to ammonia.

Unit Definition

One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

Safety & Documentation

Safety Information

Signal word 
Danger-Danger
Target organs 
Respiratory system
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

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Probiotics and Human Health

At the beginning of the 20th century, probiotics were thought to have a beneficial effect to the host by improving the intestinal microbial balance. Through his studies and observations, the Russian ...
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Keywords: AGE, Absorption, Adhesion, Amplification, Anaerobic, Antimicrobials, Cancer, Carcinogens, Cell proliferation, Chromatography, Coagulation, Detoxification, Diagnostic, Diseases, Enzyme activity, Fermentation, Gas chromatography, Gastrointestinal, High performance liquid chromatography, Inflammation, Magnetic resonance spectroscopy, Metabolism, Metabolites, Molecular biology, Nucleic acid hybridization, PAGE, Pharmaceutical, Phosphorylations, Polymerase chain reaction, Prebiotics, Probiotics, Reductions, Respiratory, Sequencing, Thin layer chromatography, Titrations

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Peer-Reviewed Papers
15

References

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