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U0631 Sigma-Aldrich

Urea

BioXtra, pH 7.5-9.5 (20 °C, 5 M in H2O)

Synonym: Carbamide, Carbonyldiamide

  • CAS Number 57-13-6

  • Linear Formula NH2CONH2

  • Molecular Weight 60.06

  •  Beilstein/REAXYS Number 635724

  •  EC Number 200-315-5

  •  MDL number MFCD00008022

  •  PubChem Substance ID 24900617

  •  NACRES NA.31

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Properties

Related Categories Biochemicals and Reagents, Building Blocks, Carbonyl Compounds, Chaotropic Agents, Chemical Synthesis,
Quality Level   200
product line   BioXtra
assay   99.0-100.5%
form   powder, crystals or pellets
impurities   ≤0.005% Phosphorus (P)
  ≤0.1% Insoluble matter
ign. residue   ≤0.1%
pH   7.5-9.5 (20 °C, 5 M in H2O)
mp   132-135 °C (lit.)
solubility   H2O: 5 M at 20 °C, clear, colorless
density   1.335 g/mL at 25 °C (lit.)
anion traces   chloride (Cl-): ≤0.05%
  sulfate (SO42-): ≤0.05%
cation traces   Al: ≤0.0005%
  Ca: ≤0.001%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Mg: ≤0.0005%
  NH4+: ≤0.05%
  Na: ≤0.005%
  Pb: ≤0.001%
  Zn: ≤0.0005%
absorption   ≤0.06 at 260 nm at 5 M
  ≤0.06 at 280 nm at 5 M
Featured Industry   Diagnostic Assay Manufacturing
SMILES string   NC(N)=O
InChI   1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
InChI key   XSQUKJJJFZCRTK-UHFFFAOYSA-N
Gene Information   human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

Description

Packaging

1 kg in poly bottle

5 kg in poly drum

500 g in poly bottle

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
YR6250000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Meet Synthia - Retrosynthesis Sofware
Protocols & Articles
Peer-Reviewed Papers
15

References

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