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U3254 Sigma-Aldrich

Monoclonal Anti-Uvomorulin/E-Cadherin antibody produced in rat

clone DECMA-1, ascites fluid, buffered aqueous solution

Synonym: Anti-E-Cadherin, Anti-LCAM

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Adhesion, Attachment, and Matrix Factors, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology,
conjugate   unconjugated
clone   DECMA-1, monoclonal
biological source   rat
application(s)   immunohistochemistry (frozen sections): suitable
  immunoprecipitation (IP): suitable
  indirect immunofluorescence: 1:1,600 using cultured MDCK cells
  microarray: suitable
  western blot: suitable
species reactivity   mouse, canine, bovine, human
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG1
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P12830
Gene Information   human ... CDH1(999)
mouse ... Cdh1(12550)

Description

General description

Monoclonal Anti-Uvomorulin/E-Cadherin (rat IgG1 isotype) is derived from the DECMA-1 hybridoma, produced by the fusion of rat myeloma cells and splenocytes from an immunized Lou rat. Uvomorulin protein was initially identified in embryonal carcinoma and is identical to E-Cadherin, liver-cell adhesion molecules (L-CAM), Cell CAM 80/120, and Activity-regulated cytoskeleton-associated protein 1 (ARC-1), each of which have been characterized in different systems. Uvomorulin/E-Cadherin has been characterized as a 120 kDa cell surface glycoprotein from which an 84 kDa fragment can be released by trypsin digestion in the presence of Ca2+.

Specificity

Monoclonal Anti-Uvomorulin/E-Cadherin was selected against the mouse cell adhesion molecule uvomorulin/E-Cadherin. The antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin that has been found to be identical to L-CAM, Cell CAM 80/120, and ARC-1. It blocks both the aggregation of mouse embryonal carcinoma cells and the compaction of pre-implantation embryos. The antibody disrupts confluent monolayers of Madin-Darby canine kidney (MDCK) epithelial cells. In indirect immunofluorescent staining of MDCK cells grown in culture, the antibody shows strong staining on the membrane of adjacent cells, after treatment with 0.5% Triton-X 100.

The antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin that has been found to be identical to L-CAM, Cell CAM 80/120, and ARC-1. The antibody may be used for studies of embryonal development, cell-cell interactions of cultured cells, and localization of uvomorulin/E-cadherin in immunoblotting or immunohistochemical assays.

Immunogen

mouse embryonal carcinoma cell line PCC4 Aza R1.

Application

Monoclonal Anti-Uvomorulin/E-Cadherin has been used in immunofluorescence, immunoblotting, immunoprecipitation, immunohistochemistry, macromolecule permeability assay and agglomeration of two embryoid body assay.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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