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U4883 Supelco

Urea

8 M (after reconstitution with 16 mL high purity water)

Synonym: Carbamide, Carbonyldiamide, Urea solution

  • CAS Number 57-13-6

  • Linear Formula NH2CONH2

  • Molecular Weight 60.06

  •  Beilstein/REAXYS Number 635724

  •  EC Number 200-315-5

  •  MDL number MFCD00008022

  •  PubChem Substance ID 24900649

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Properties

Related Categories Biochemicals and Reagents, Buffers and Reagents, Buffers, Reagents, & Stains, Chaotropic Agents, Denaturation,
Quality Level   100
form   powder
concentration   8 M (after reconstitution with 16 mL high purity water)
solubility   H2O: soluble (1 vial plus 16 mL)
SMILES string   NC(N)=O
InChI   1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
InChI key   XSQUKJJJFZCRTK-UHFFFAOYSA-N

Description

General description

Urea is a chaotropic agent and is used for protein denaturation. It disturbs the hydrogen bonds in the secondary, tertiary and quaternary structure of proteins. It can also disturb hydrogen bonding present in DNA secondary structure.

Application

Useful for solubilizing and denaturing proteins in preparation for 2D-PAGE, enzymatic digests, IMAC Chromatography, and related procedures.
Urea has been used for protein sample preparation for reverse-phase nanospray liquid chromatography-tandem mass spectrometry.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
YR6250000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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