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U5378 Sigma

Urea

powder, BioReagent, for molecular biology, suitable for cell culture

Synonym: Carbamide, Carbonyldiamide

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Properties

Related Categories Biochemicals, Biochemicals and Reagents, Chaotropic Agents, Core Bioreagents, Denaturation,
grade   for molecular biology
InChI Key   XSQUKJJJFZCRTK-UHFFFAOYSA-N
product line   BioReagent
assay   ≥98%
form   powder
color   white
mp   132-135 °C(lit.)
solubility   H2O: soluble 480 mg/mL clear, colorless (8M)
density   1.335 g/mL at 25 °C(lit.)
anion traces   chloride (Cl-): ≤5 ppm
cation traces   Cu: ≤1 ppm
  Fe: ≤1 ppm
  Pb: ≤2 ppm
absorption   A6M/260 ≤0.06
  A6M/280 ≤0.06
suitability   suitable for cell culture
foreign activity   DNase, NICKase, RNase and protease, none detected
Gene Information   human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

Description

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

• Urea has been used in protein resuspension buffer and to denature proteins.
• It has been used for the isolation of proteins via urea-containing polyacrylamide gels.
• It has been used to study the conformation of unfolded proteins under different concentrations of urea and different pH.

Packaging

1 kg in poly bottle

100, 500 g in poly bottle

5 kg in poly drum

Price and Availability


LC-MS Grade Solvents and Reagents

Bulk Chemicals

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
YR6250000
Protocols & Articles

Articles

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation

Peer-Reviewed Papers
15

References

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