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  • U5383 - Anti-Ubiquitin C-terminal Hydrolase L1 (TS-21) antibody produced in rabbit

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U5383 Sigma-Aldrich

Anti-Ubiquitin C-terminal Hydrolase L1 (TS-21) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-UCH-L1

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Parkinson’s Disease Research, Antibodies to Protein Degradation,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1:1,000-1:2,000 using cytosolic fraction (S1) of mouse brain or a whole cell extract of human lung carcinoma A549 cell line
  western blot: 1:5,000-1:10,000 using cytosolic fraction (S1) of rat brain
species reactivity   mouse, human, rat
mol wt   antigen 27 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P09936
Gene Information   human ... UCHL1(7345)
mouse ... Uchl1(22223)
rat ... Uchl1(29545)

Description

Immunogen

synthetic peptide corresponding to amino acids 56-76 located near the N-terminus of rat UCH-L1, conjugated to KLH. This sequence is identical in human, mouse, bovine, porcine, and guinea pig UCH-L1. No homology is found with other known UCH-L isoforms.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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U5258 Anti-Ubiquitin C-terminal Hydrolase L1 (RA-15) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

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