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U6504 Sigma-Aldrich

Urea

for electrophoresis

Synonym: Carbamide, Carbonyldiamide

  • CAS Number 57-13-6

  • Linear Formula NH2CONH2

  • Molecular Weight 60.06

  •  Beilstein/REAXYS Number 635724

  •  EC Number 200-315-5

  •  MDL number MFCD00008022

  •  PubChem Substance ID 24900435

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Properties

Related Categories Biochemicals and Reagents, Buffers and Reagents, Buffers, Reagents, & Stains, Chaotropic Agents, Core Bioreagents,
grade   for electrophoresis
InChI Key   XSQUKJJJFZCRTK-UHFFFAOYSA-N
assay   ≥98%
form   powder or crystals
impurities   ≤0.005% Ammonia
mp   132-135 °C (lit.)
solubility   H2O: 100 mg/mL
density   1.335 g/mL at 25 °C (lit.)
cation traces   Fe: ≤5 ppm
  Pb: ≤5 ppm
Gene Information   human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

Description

Packaging

1 kg in poly bottle

500 g in poly bottle

Application

A chaotropic agent used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis.

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
YR6250000

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles
Peer-Reviewed Papers
15

References

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