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  • U7008 - Anti-UCH-L3 antibody produced in rabbit

U7008 Sigma

Anti-UCH-L3 antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Ubiquitin C-terminal esterase L3



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Protein Degradation, Antibodies to Ubiquitin, Ubiquitin-like and Proteasome Proteins,
species reactivity   human, rat, mouse
application(s)   western blot: 1-2 μg/mL using mouse and rat brain extracts (S1 fraction) and HEK-293T cells expressing human UCH-L3
clone   polyclonal
concentration   ~1.5 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~30 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... UCHL3(7347)
mouse ... Uchl3(50933)
rat ... Uchl3-ps1(114094)
biological source   rabbit
conjugate   unconjugated



synthetic peptide correspopnding to amino acids 182-200 of human UCH-L3 conjugated to KLH. This sequence is highly conserved (one amino acid change) in mouse and rat UCH-L3.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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U2133 Ubiquitin C-terminal Hydrolase-L3
U7133 Anti-UCH-L3 (C-terminal) antibody produced in rabbit, ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

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