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  • U7883 - Monoclonal Anti-USP25 antibody produced in mouse

U7883 Sigma-Aldrich

Monoclonal Anti-USP25 antibody produced in mouse

~1.0 mg/mL, clone U25.7, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-USP21, Anti-Ubiquitin specific peptidase 25

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Protein Degradation, Antibodies to Ubiquitin, Ubiquitin-like and Proteasome Proteins,
conjugate   unconjugated
clone   U25.7, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  indirect ELISA: suitable
  western blot: 1-2 μg/mL using total extract of HEK-293T cells expressing USP25.
species reactivity   human, rat, mouse
mol wt   antigen ~126 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2a
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   Q9UHP3
Gene Information   human ... USP25(29761)
mouse ... Usp25(30940)
rat ... Usp25(304150)


General description

Monoclonal Anti-USP25 (mouse IgG2a isotype) is derived from the hybridoma U25.7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human USP25. USP25 (ubiquitin-specific protease 25) is a member of the ubiquitin processing proteases (UBP) family. It is located on the gene-poor region of 21q11.2 human chromosome.


Monoclonal Anti-USP25 antibody has been used in:
• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

USP25 (ubiquitin-specific protease 25) is a target for sumoylation. It takes place within the two ubiquitin interaction motifs (UIM) of USP25 that are required for efficient hydrolysis of Ub (ubiquitin), and thus impairs USP25 activity as a result of reduced affinity to Ub chains. Overexpression of USP25 has been observed in Down syndrome fetal brain.

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers


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