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V1014 Sigma-Aldrich

Anti-Vascular Endothelial Growth Factor Receptor 2 antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-Flk-1, Anti-VEGF R2

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Properties

Related Categories Alphabetical Index, Angiogenesis, Antibodies, Antibodies against Cytokines/Growth Factors/Chemokines for Stem Cell Biology, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology,
conjugate   unconjugated
clone   polyclonal
biological source   goat
application(s)   flow cytometry: 3-10 μg/mL (106 cells)
  immunohistochemistry: 5-15 μg/mL using cells and tissues
  neutralization: suitable
  western blot: 0.1-0.2 μg/mL
species reactivity   mouse
form   lyophilized powder
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   P35918
Gene Information   mouse ... Kdr(16542)

Description

General description

Vascular endothelial growth factors receptors (VEGF Rs) are receptor tyrosine kinases that bind to VEGF that plays important role in embryonic vasculogenesis, angiogenesis and homeostasis. VEGF R2 has the ability to form heterodimers with VEGF R1 and VEGF R3. The binding of VEGF to VEGF R2 results in activation of tyrosine amino acids of the cytoplasmic tail of the receptor. This interaction results in the activation of MAPK, PI3K, PKC, FAK and Src kinase pathways. The biological roles of VEGF R2 include in epithelial cell growth, proliferation, survival, migration and vascular permeability and angiogenesis. Due to its role in promoting migration and angiogenesis, VEGF R2 has been implicated in breast and prostate cancer
Anti-Vascular Endothelial Growth Factor Receptor-2 recognizes mouse VEGF R2.

Specificity

The antibody has the ability to neutralize the biological activity of recombinant mouse VEGF R2. It demonstates approximately 40% cross-reactivity with recombinant human VEGF R2, 2% cross-reactivty with recombinant mouse VEGF R1, and no cross-reactivity with recombinant human VEGF R1 and VEGF R3.

Immunogen

purified recombinant mouse VEGF R2 extracellular domain expressed in NSO cells.

Application

Anti-Vascular Endothelial Growth Factor Receptor 2 antibody may be used in immunoblotting at a working concentration of 0.1-0.2 μg/ml. For flow cytometry the recommended concentration is 3-10 μg/ml. The immunohistochemistry of cells and tissues, a working concentration of 5-15 μg/ml may be used. For neutralization reactions, ND50 of 0.1-0.3 μg/ml is recommended.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin


Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Cancer Stem Cells: New Targets for Cancer Therapy

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Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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