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  • V4758 - Monoclonal Anti-Vascular Endothelial Growth Factor antibody produced in mouse

V4758 Sigma

Monoclonal Anti-Vascular Endothelial Growth Factor antibody produced in mouse

clone 26503, purified immunoglobulin, lyophilized powder

Synonym: Anti-VEGF



Related Categories Alphabetical Index, Angiogenesis, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
species reactivity   human, primate
application(s)   capture ELISA: 2-8 μg/mL
  immunohistochemistry: 8-25 μg/mL
  neutralization: suitable
  western blot: 1-2 μg/mL
clone   26503, monoclonal
antibody form   purified immunoglobulin
form   lyophilized powder
isotype   IgG2b
storage temp.   −20°C
Gene Information   human ... VEGFA(7422), VEGFB(7423), VEGFC(7424)
biological source   mouse
conjugate   unconjugated



purified recombinant Sf21-derived human VEGF (165 aa isoform).

General description

The antibody will neutralize the biological activity of recombinant human VEGF165. It also recognizes VEGF121.

Physical form

Lyophilized from a 0.2 μm solution in phosphate buffered saline containing carbohydrates.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

The vascular endothelial growth factor family consists of five members, VEGF-A to VEGF-E. They play important roles in important role in embryonic vasculogenesis, angiogenesis and homeostasis. The interaction between VEGFs and their respective receptors results in the activation of MAPK, PI3K, PKC, FAK and Src kinase pathways.

Price and Availability

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Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


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