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V5265 Sigma-Aldrich

Crystal violet solution

1%, aqueous solution

  • CAS Number 548-62-9

  • Empirical Formula (Hill Notation) C25H30ClN3

  • Molecular Weight 407.98

  •  Beilstein/REAXYS Number 3580948

  •  MDL number MFCD00011750

  •  PubChem Substance ID 329829057

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Properties

Related Categories C, Cell Biology, Hematology and Histology, Routine Histology Stains, Stains & Dyes, A to Z,
Quality Level   200
form   aqueous solution
concentration   1%
application(s)   hematology: suitable
  histology: suitable
Amax   0.36-0.44 at 589-594 nm
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   [Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C
InChI   1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
InChI key   ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Description

General description

Crystal violet, also known as gentian violet, is a protein dye and is basic in nature. It stains the fatty portions of sebaceous sweat a deep purple color. Crystal violet can also be used to enhance bloody fingerprints. This dye is harmful if inhaled, swallowed or absorbed through skin, contact may cause cancer, severe eye irritation in human beings.

Application

Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.

Crystal violet solution has been used as a stain in cell proliferation assays, migration assays and Boyden chamber assay.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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