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  • V5764 - Anti-Vesicular GABA Transporter antibody produced in rabbit

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V5764 Sigma-Aldrich

Anti-Vesicular GABA Transporter antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-VGAT

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies to Drug Transport Proteins, Biochemicals and Reagents,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   microarray: suitable
  western blot: 2-4 μg/mL using mouse brain and rat brain extracts (S1 fraction)
species reactivity   mouse, rat
mol wt   antigen ~55 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
packaging   pkg of 200 μL
antibody form   affinity isolated antibody
antibody product type   primary antibodies
potency   2-4 mg per mL effective concentration (determined by immunoblotting, using mouse brain and rat brain extracts)
storage condition   avoid repeated freeze/thaw cycles
concentration   ~1.0 mg/mL
UniProt accession no.   Q9H598
O35458
O35633
Gene Information   human ... SLC32A1(140679)
mouse ... Slc32a1(22348)
rat ... Slc32a1(83612)

Description

General description

Vesicular GABA transporter (VGAT), also known as solute carrier family 32 member 1 (SLC32A1) or vesicular inhibitory amino acid transporter (VIAAT), is encoded by the gene localized on human chromosome 20q11.23. The encoded protein is highly expressed in both γ-aminobutyric acid -ergic (GABAergic) and glycinergic nerve endings.

Immunogen

synthetic peptide corresponding to amino acids 1-20 located at the N-terminus of mouse VGAT. This sequence is identical in rat VGAT and highly conserved (single a.a. substitution) in human VGAT.

Application

Anti-Vesicular GABA Transporter antibody produced in rabbit has been used in:
•  immunohistochemistry
•  western blot
•  immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Biochem/physiol Actions

Vesicular GABA transporter (VGAT) plays a vital role in loading of presynaptic vesicles with glycine and γ-aminobutyric acid (GABA). VGAT is precisely involved in packing GABA into synaptic vesicles. In addition, it also has a crucial role in inhibitory synaptic transmission. Genetic variations in the gene leads to hyperekplexia.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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