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  • V5764 - Anti-Vesicular GABA Transporter antibody produced in rabbit

V5764 Sigma-Aldrich

Anti-Vesicular GABA Transporter antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-VGAT



Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies to Drug Transport Proteins, Biochemicals and Reagents,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   microarray: suitable
  western blot: 2-4 μg/mL using mouse brain and rat brain extracts (S1 fraction)
species reactivity   mouse, rat
mol wt   antigen ~55 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
packaging   pkg of 200 μL
antibody form   affinity isolated antibody
antibody product type   primary antibodies
potency   2-4 mg per mL effective concentration (determined by immunoblotting, using mouse brain and rat brain extracts)
storage condition   avoid repeated freeze/thaw cycles
concentration   ~1.0 mg/mL
UniProt accession no.   Q9H598
Gene Information   human ... SLC32A1(140679)
mouse ... Slc32a1(22348)
rat ... Slc32a1(83612)


General description

Vesicular GABA transporter (VGAT), also known as solute carrier family 32 member 1 (SLC32A1) or vesicular inhibitory amino acid transporter (VIAAT), is encoded by the gene localized on human chromosome 20q11.23. The encoded protein is highly expressed in both γ-aminobutyric acid -ergic (GABAergic) and glycinergic nerve endings.


synthetic peptide corresponding to amino acids 1-20 located at the N-terminus of mouse VGAT. This sequence is identical in rat VGAT and highly conserved (single a.a. substitution) in human VGAT.


Anti-Vesicular GABA Transporter antibody produced in rabbit has been used in:
•  immunohistochemistry
•  western blot
•  immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Biochem/physiol Actions

Vesicular GABA transporter (VGAT) plays a vital role in loading of presynaptic vesicles with glycine and γ-aminobutyric acid (GABA). VGAT is precisely involved in packing GABA into synaptic vesicles. In addition, it also has a crucial role in inhibitory synaptic transmission. Genetic variations in the gene leads to hyperekplexia.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis


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Milli-Q® Water Purification Solutions
Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers


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