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V6389 Sigma-Aldrich

Monoclonal Anti-Vimentin antibody produced in mouse

clone V9, purified from hybridoma cell culture

Synonym: Anti-Vimentin antibody, Mouse monoclonal

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Alzheimer′s Disease, Antibodies for Cardiac Stem Cells,
conjugate   unconjugated
clone   V9, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 25-50 μg/mL using human tissue
  microarray: suitable
  western blot: 0.2-0.5 μg/mL using human fibroblasts HS-68
species reactivity   bovine, feline, chicken, dog, gerbil, rat, monkey, hamster, horse, pig, human
mol wt   antigen mol wt ~58 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified from hybridoma cell culture
isotype   IgG1
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   P08670
Gene Information   human ... VIM(7431)
rat ... Vim(81818)

Description

General description

Vimentin is encoded by a single-copy gene mapped to human chromosome 10p13. 53kDa vimentin protein belongs to type III intermediate filament (IF) family and is specifically expressed in normal mesenchymal cells. Vimentin protein with 466 amino acids is characterized with a highly conserved α-helical “rod” domain that is flanked by non-α--helical amino- terminal “head” and carboxy-terminal “tail” domain.

Specificity

The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas.

Immunogen

pig eye lens vimentin.

Application

Monoclonal Anti-Vimentin antibody produced in mouse has been used in immunohistochemical analysis and western blot assay.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Vimentin plays a vital role in maintaining the structural integrity of the cells. Overexpression of vimentin is observed in prostate cancer, breast cancer, endometrial cancer, central nervous system (CNS) tumors, malignant melanoma and gastrointestinal tumors including pancreatic, colorectal and hepatic cancers. Thus, vimentin can act as a target for developing therapeutics for the treatment of cancer. Vimentin plays a crucial role in the vascular endothelial growth factor receptor-1 (VEGFR-1)-induced activation of the protein kinase G (PKG) 1 signaling pathway, stimulating regression of cardiomyocyte hypertrophy. Vimentin filaments are implicated in various physiological process including migration, maintenance of cell shape and tolerance of mechanical stress of mesenchymal cells. Vimentin interacts with LARP6 (La ribonucleoprotein domain family member 6) and stabilizes type I collagen mRNAs and might play a vital role in the development of tissue fibrosis. Vimentin plays an essential role in the maintenance of lens integrity, therefore, mutation of the vimentin gene causes dominant and pulverulent cataract.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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