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V7259 Sigma-Aldrich

Vascular Endothelial Growth Factor human

VEGF, recombinant, expressed in E. coli, powder, suitable for cell culture

Synonym: VEGF



Related Categories Angiogenesis, Angiogenic Factors, Cancer Research, Cell Biology, Cell Culture,
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (SDS-PAGE)
form   powder
potency   1-10 ng/mL ED50/EC50
quality   endotoxin tested
mol wt   protein 38.2 kDa (as homodimer)
packaging   pkg of 10 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   ≤0.1 EU/μg
color   white
solubility   water: soluble
suitability   suitable for cell culture
UniProt accession no.   P15692
storage temp.   −20°C
Gene Information   human ... VEGFA(7422)


General description

Vascular Endothelial Growth Factor (VEGF) gene is localized to human chromosome 6p21.3, and is thought to encode four different isoforms.


Vascular Endothelial Growth Factor (VEGF) has been used for-
• the determination of oligodendrocyte precursor cells (OPCs) and luteal cell proliferation/survival
• inducing proliferation of epithelial cell clusters (ECCs) to be transplanted into mice

Biochem/physiol Actions

Vascular Endothelial Growth Factor (VEGF), also known as vasculotropin, is an angiogenic growth factor, which is heat and acid stable. VEGFs stimulate endothelial cell growth, angiogenisis, and capillary permeability. VEGF is a secreted homodimeric, heparin-binding glycoprotein,1 which has an isoelectric point of 8.5. VEGF promotes the growth of endothelial cells isolated from bovine adrenal cortex, cerebral cortex, fetal and adult aorta, and human umbilical vein. The target cell specificity of VEGF is restricted to vascular endothelial cells. VEGF has no mitogenic effect on cultured corneal endothelial cells, vascular smooth muscle cells, BHK-12 fibroblasts, keratinocytes, human sarcoma cells, or lens epithelial cells. A variety of human tumor cell lines including sarcoma and carcinoma cells show a 3.7 kb RNA transcript that hybridizes with the VEGF probe in a Northern blot. Mouse sarcoma 180 cells express the VEGF mRNA and secrete a VEGF-like mitogen.

VEGF recombinant protein might have potential as a therapeutic agent in treatment-resistant disorders, where it can facilitate endothelial cell growth and vessel formation.

Physical properties

Monomer consists of 165 amino acid residues, approx. mol. weight 19.2 kDa

Physical form

Lyophilized from a sterile filtered buffered solution with no additives.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


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Protocols & Articles


Growth Factors in Stem Cell Biology

Stem cell biology researchers use suitable growth factors to trigger proliferation, differentiation and/or migration of stem cells. Embryonic pluripotent stem cells can differentiate into three germ ...
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Predictive Models for Neuroscience Research

For neuroscientists, the quest for truly predictive neural models has its challenges given the brain is a very complex organ comprised of many diverse cell types, complex cell-tocell interactions and...
Keywords: Apoptosis, Cell culture, Cell proliferation, Central Nervous System, Deposition, Diseases, Dopamine agents, Drug discovery, Environmental, Filtration, Gene expression, Genetic, Growth factors, Neuroscience, Reductions, Schizophrenia, Sequencing, Sterilizations, Transfection

Vascular Endothelial Growth Factor (VEGF)

Background VEGF Pathway    Ligands    Receptors VEGF – Signal Transduction Factors Regulating VEGF and VEGFR Expression VEGFR in Human Diseases Conclusion
Keywords: Angiogenesis, Apoptosis, Cancer, Cell proliferation, Diseases, Gene expression, Growth factors, Infrared spectroscopy, Ligands, Transcription, Transduction

Vascular Endothelial Growth Factors (VEGF)

Vascular endothelial growth factor (VEGF) is a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. Originally known simpl...
Jennifer Fries
BioFiles 2009, 4.5, 26.
Keywords: Angiogenesis, Gene expression, Growth factors, Inflammation, Ligands

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Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

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