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V9131 Sigma

Monoclonal Anti-Vinculin antibody produced in mouse

clone hVIN-1, ascites fluid

Synonym: Anti Vinculin, Vinculin Antibody - Monoclonal Anti-Vinculin antibody produced in mouse - Tested Applications: western blot extract of human fibroblasts 1:200,immunohistochemistry (frozen sections),indirect immunofluorescence cultured human fibroblasts 1:400, Vinculin Antibody

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cell and Organelle Proteins,
species reactivity   canine, mouse, chicken, rat, frog, bovine, human, turkey
application(s)   immunohistochemistry (frozen sections): suitable
  indirect immunofluorescence: 1:400 using cultured human fibroblasts
  western blot: 1:200 using extract of human fibroblasts
clone   hVIN-1, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 116 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)
biological source   mouse
conjugate   unconjugated
Featured Industry   Research Pathology

Description

Immunogen

purified human vinculin from uterus.

General description

Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions that functions as one of several interacting proteins involved in anchoring F-actin to the membrane. The sequences of chicken, nematode and human vinculin have been determined.
A sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the β-subunit of integrin binds to talin. Talin binds to vinculin that interacts with α-actinin and possibly with itself. Since α-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions and the membrane-associated dense bodies of smooth muscle. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.

Specificity

Monoclonal Anti-Vinculin specifically stains vinculin at cell-cell and cell-substrate contacts in tissue and cultured cells using indirect immunofluorescent labeling. The antibody reacts with the 116 kDa vinculin band in immunoblotting. The product reacts with vinculin of many species. Good reactivity is obtained with human, bovine, chicken, dog, rat, mouse, turkey, and Xenopus. The antibody shows cross reactivity with smooth muscle metavinculin.

Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.

Application

Monoclonal Anti-Vinculin may be used for the localization of human and animal vinculin using various immunoassays including immunocytochemical localization by means of immunofluorescence labeling of cultured cells, immunohistological staining of frozen tissue sections, and immunoblotting.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Improving Reproducibility: Best Practices for Antibodies

In the midst of beeping lab timers, presentations and grant deadlines, it is easy to take for granted the quality of lab reagents.
Keywords: Cancer, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatography, Clinical, Degradations, Electrophoresis, Enzyme-linked immunosorbent assay, Gene expression, High performance liquid chromatography, Immunohistochemistry, Immunoprecipitation, Liquid chromatography mass spectrometry, Mass spectrometry, Mass spectrometry detection, Pharmaceutical, Purification, Sequencing, Titrations, Western blot

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Peer-Reviewed Papers
15

References

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Description

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V9264 Anti-Vinculin antibody, Mouse monoclonal, clone hVIN-1, purified from hybridoma cell culture
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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