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  • V9263 - Monoclonal Anti-Vascular Cell Adhesion Molecule 1 antibody produced in mouse

V9263 Sigma-Aldrich

Monoclonal Anti-Vascular Cell Adhesion Molecule 1 antibody produced in mouse

~1 mg/mL, clone 1.4C3, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-VCAM-1



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Stem Cell Biology,
conjugate   unconjugated
clone   1.4C3, monoclonal
biological source   mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL using human tonsil tissue
  immunohistochemistry (frozen sections): 2-4 μg/mL using human tonsil tissue
  western blot: suitable
species reactivity   human
mol wt   antigen 110 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   P19320
Gene Information   human ... VCAM1(7412)


General description

VCAM-1(Vascular Cell Adhesion Molecule-1) belongs to immunoglobulin-related gene superfamily of adhesion molecules and is a 110kD cell surface integral membrane glycoprotein. It is expressed mainly by cytokine-activated endothelium and plays a pivotal role in numerous immunological and inflammatory responses. Monoclonal anti-vascular cell adhesion molecule 1 antibody can be used in immunohistochemistry for formalin-fixed, paraffin-embedded tissue sections. Mouse anti-vascular cell adhesion molecule 1 antibody reacts specifically with human vascular cell adhesion molecule-1 (CD106 or INCAM-110).


stimulated HUVEC cells and mouse NS1 cells.


Monoclonal anti-vascular cell adhesion molecule 1 antibody can be used in immunoblotting and western blotting.

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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