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VRE100 Sigma-Aldrich

Viral RNA Extraction Buffer

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One Step RT-qPCR Compatible, Optimized for Viral RNA Detection in Saliva

Synonym: For use with Reverse Transcription-qPCR, Viral Lysis Solution for RT-qPCR, Viral RNA Extraction

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Properties

Related Categories Application Index, Biochemicals and Reagents, DNA & RNA Purification, Enzymes, Inhibitors, and Substrates, Molecular Biology,
Quality Level   200
grade   Molecular Biology
form   liquid
concentration   4X
application(s)   LAMP: suitable
  PCR: suitable
  RT-PCR: suitable
  qPCR: suitable
shipped in   wet ice
storage temp.   −20°C

Description

General description

The Viral RNA Extraction Buffer has been optimized to provide rapid, room-temperature lysis of SARS-CoV-2 viral particles in saliva. The buffer comes as a 4x working solution that can be mixed with virus-containing samples in a qRT-PCR plate. Upon 5 minute incubation at room temperature, viral particles are lysed and RNA is stabilized such that samples are ready for direct analysis via RT-qPCR with no additional cleanup step. The Viral RNA Extraction Buffer is compatible with several one-step RT-qPCR kits, including Sigma’s Quantitative RT-PCR ReadyMix (QR0200). Removal of precipitates in saliva via centrifugation prior to testing is recommended for optimal sensitivity.

Application

The Viral RNA Extraction Buffer is broadly compatible with most nucleic acid detection methods and does not reduce signal when used at working concentration in qRT-PCR. qRT-PCR compatibility has been demonstrated in a 4-fluor multiplex setting consisting of Cy5, TxRed, FAM, and HEX.

RT-qPCR compatibility has been verified with several one-step RT-qPCR kits:
• Quantitative RT-PCR ReadyMix (Sigma-Aldrich, catalog QR0200)
• KAPA PROBE FAST One-Step Universal (Sigma-Aldrich, catalog KK4752)
• KiCqStart® One-Step Probe RT-qPCR ReadyMix (Sigma-Aldrich, catalog KCQS07)
• TaqPathTM 1-Step Multiplex Master Mix (Thermo Fisher, catalog A28525)

The Viral RNA Extraction Buffer is also compatible with colorimetric LAMP assays.

Features and Benefits

• Extract viral RNA without cleanup
• Directly compatible with qRT-PCR and LAMP assays
• Compatible with saliva or saline samples
• More sensitive than other direct saliva-based RNA detection methods

Legal Information

KiCqStart is a registered trademark of Qiagen Beverly Inc.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

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