• USA Home
  • W0890 - Anti-WASP antibody produced in rabbit

W0890 Sigma

Anti-WASP antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous glycerol solution

Synonym: Anti-Wiskott-Aldrich Syndrome Protein



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cell and Organelle Proteins,
species reactivity   mouse, human
application(s)   immunoprecipitation (IP): 4 μg using 0.5 mg of human Jurkat and Raji and murine BAF-3 RIPA lysates
  western blot: 2 μg/mL using RIPA lysates from human Jurkat and Raji and murine BAF-3 cells
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous glycerol solution
mol wt   antigen mol wt 65 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... WAS(7454)
mouse ... Was(22376)
biological source   rabbit
conjugate   unconjugated



GST fusion protein corresponding to residues 1-379 of human WASP

Antigen Background

WASP interacts with the GTPase CdC42 and seems to provide a link between Cdc42 and the actin cytoskeleton.

Physical form

Solution in 0.07 M Tris-glycine, pH 7.4, containing 0.105 M NaCl, 30% glycerol and 0.035% sodium azide


WASP may migrate on SDS-PAGE at an apparent molecular weight ranging from 59-75 kDa. In some cell lines, an unknown band (85 kDa) is seen. Cross-reactivity with N-WASP, a homologue protein isolated from brain, has not been determined.

Preparation Note

Purified using protein A

Price and Availability

Antibody Explorer

Explore with Confidence
Safety & Documentation

Safety Information

WGK Germany  1
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?