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W2266 Sigma-Aldrich

Anti-Wnt-4 antibody produced in goat

affinity isolated antibody, lyophilized powder

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies for Neural Stem Cells,
conjugate   unconjugated
clone   polyclonal
biological source   goat
application(s)   indirect ELISA: 0.5-1 μg/mL using rmWnt-4 peptide
  western blot: 0.1-0.2 μg/mL using mammalian cell-expressed mouse Wnt-4 protein
species reactivity   mouse
form   lyophilized powder
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   P22724
Gene Information   mouse ... Wnt4(22417)

Description

Specificity

Shows no cross-reactivity with other 6 histidine containing peptides.

Immunogen

purified E. coli-derived, recombinant mouse Wnt-4 peptide consisting of amino acid residues 37-76 and 222-295 joined by a linker. The peptide has six histidine residues added at the C-terminus.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Cancer Stem Cells: New Targets for Cancer Therapy

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Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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