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  • W2519 - Anti-WIPI-1 (N-terminal) antibody produced in rabbit

W2519 Sigma

Anti-WIPI-1 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-ATG18, Anti-WD repeat domain phosphoinositide interacting 1, Anti-WD40 repeat protein Interacting with phosphoInositides of 49kDa, Anti-WIPI-1α, Anti-WIPI49



Related Categories Alphabetical Index, Antibodies, Antibodies for Autophagy, Antibodies for Cell Biology, Primary Antibodies,
species reactivity   human
application(s)   western blot: 2.5-5.0 μg/mL using whole extract of human G-361 cells.
clone   polyclonal
concentration   ~1.0 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~49 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... WIPI1(55062)
mouse ... Wipi1(52639)
rat ... Wipi1(303630)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acids 46-61 of human WIPI-1, conjugated to KLH. The corresponding sequence is identical in mouse and rat.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

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Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

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