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W4394 Sigma-Aldrich

Withaferin A

≥95% (HPLC)

Synonym: 5,6-Epoxy-4,27-dihydroxy-1-oxowitha-2,24-dienolide, Withaferine A

  • CAS Number 5119-48-2

  • Empirical Formula (Hill Notation) C28H38O6

  • Molecular Weight 470.60

  •  MDL number MFCD10687098

  •  PubChem Substance ID 329830709

  •  NACRES NA.77

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Properties

Related Categories Angiogenesis, Angiogenesis Inhibitors, Cancer Research, Cell Biology
InChI Key   DBRXOUCRJQVYJQ-CKNDUULBSA-N
assay   ≥95% (HPLC)
mp   252-253 °C
storage temp.   2-8°C

Description

Application

Withaferin A was used to treat HEK293 cells to study its effect on cystic fibrosis inflammation.

Biochem/physiol Actions

Withaferin A is a steroidal lactone that is isolated from the plant Withania somnifera. It has potent anti-inflammatory properties as it inhibits the activation of NF-κ B signaling pathway. The anti-tumor activity of Withaferin A is due to its ability to alter cytoskeletal architecture by binding annexin II and disrupting F actin cross-links. Withaferin A also inhibits angiogenesis by binding to vimentin and F-actin.

Steroidal lactone that exhibits cytotoxicity towards tumor cells. Protective effects attributed to anti-lipid peroxidative, antioxidant and detoxifying functionality.

Preparation Note

Withaferin A yields a clear, colorless solution in methanol at 1 mg/ml.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Flash Point(F) 
438.8 °F
Flash Point(C) 
226 °C

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the solution stability of Product W4394, Withaferin A?
Sigma-Aldrich does not test the Product W4394, Withaferin A, for solution stability.Information in our files indicates that although it is best to use freshly prepared solution when possible. However, if stock solutions must be stored, we would recommend they be storedin frozen aliquots at-20°C or below for about one month.
How does one solubilizeProduct W4394, Withaferin A?
Sigma-Aldrich testsProduct W4394, Withaferin A, for solubility using methanol at a concentration of1 mg/mL. Information in our files indicates that it is also soluble in DMSO and ethanol.
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Protocols & Articles

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Peer-Reviewed Papers
15

References

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