• USA Home
  • WGA1 - GenomePlex® Whole Genome Amplification (WGA) Kit

EMAIL THIS PAGE TO A FRIEND
WGA1 Sigma-Aldrich

GenomePlex® Whole Genome Amplification (WGA) Kit

Kit for whole genome amplification from a variety of DNA sources including FFPE tissue

  •  NACRES NA.55

Purchase

Description

General description

WGA is suitable for use with genomic DNA, whole blood, buffy coats, buccal swabs, and cultured cells. Amplification requires only a small amount of starting material (1 μL blood, single cheek swab, or 10 ng of gDNA), which after PCR produces a yield of 5 - 10 μg per reaction. The amplified DNA can be purified and stored or used for downstream analyses including ABI′s TaqMan® assays, microsatellite analysis, or sequencing. GenomePlex gives a complete representation of the entire genome with no detectable allele bias.

GenomePlex Whole Genome Amplification (WGA) Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.

Application

GenomePlex® Whole Genome Amplification (WGA) Kit has been used to amplify input and chromatin immunoprecipitation (ChIP) DNA.

Features and Benefits

• Starting material: 10 ng
• Expected yield: 5 - 10 μg
• Time required: ~3 hours
• Universal primers
• Whole genome representation with no detectable bias
• Increased accuracy in amplification produces no amplicon in the negative control reactions
• Choose from a variety of DNA sources: whole blood, buccal swab, blood card, plant, soil, & formalin-fixed paraffin-embedded tissue (FFPE)

Other Notes

The sequences of the universal primers provided in this kit are considered proprietary.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

GenomePlex is a registered trademark of Takara Bio USA, Inc.

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Kit component also available separately

Description

Product #

Add to Cart

Water, Nuclease-Free Water, for Molecular Biology SDS W4502
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Frequently Asked Questions

What is the major difference between WGA1 and WGA2?
Functionally WGA1 and WGA2 are identical. The only difference between the two kits is that WGA2 is supplied with the WGA polymerase.
If starting with fragmented DNA, what is the smallest size fragment which can be successfully amplified with WGA1, GenomePlex® Amplification Kit, and do I still need to do the fragmentation step?
The kit works best for fragments 400 bp and larger. When starting with fragmented DNA, we recommend: (1) skipping the fragmentation heat step, although the buffer should be added, and (2) increasing the PCR cycles from 14 to 20.
Can I use WGA1, GenomePlex® Amplification Kit to amplify DNA from a single cell?
Using this kit to amplify DNA from a single cell is not recommended. We recommend using the GenomePlex Single Cell WGA Kit (WGA4) for such application. WGA4 was developed for use with single cells and includes an optimized cell lysis protocol which has been incorporated into the fragmentation step.
Can I amplify single stranded DNA with Product WGA, GenomePlex® Amplification Kit?
When starting with single stranded starting materials, we recommend (1) skipping the fragmentation heat step, although the buffer should be added and (2) increasing the PCR cycles from 14 to 20. Note that if the ssDNA is actually cDNA from polyadenylated RNA, the kit will likely not give good representation of the input material, as the poly(T) ends constitute a large, non-random fraction.
Is the WGA1 GenomePlex®Amplicification Kit polymerase compatible with TA cloning?
WGA polymerase is compatible with TA cloning with the following alteration to the PCR step: Be sure to include a 7 to 30 minute extension at 72°C after the last cycle to ensure that all PCR products are full length and 3´ adenylated.
What is the major difference between WGA1 and WGA2 kits?
Functionally, WGA1 and WGA2 kits are identical. The only difference between the two kits is that WGA2 is supplied with the WGA polymerase.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions
Protocols & Articles

Articles

Amplification of genome-representative DNA from limited sources with GenomePlex® WGA technology for use in genetic alterations studies

Modern technologies developed in recent years have prompted new and exciting approaches to support biomedical research. The ability to study the entire human genome using limited sources of genomic m...
Victoria Rusakova & Lukasz Nosek
Sigma-Aldrich Biotechnology
Keywords: Amplification, Clinical, DNA purification, Diagnostic, Diseases, Functional genomics, Genetic, Genetics, Genomics, In Situ hybridization, Microarray Analysis, Nucleic acid hybridization, Polymorphisms, Purification, Sequencing

Histone Modification and Chromatin Remodeling

Gene expression is governed by complex mechanisms including transcription factor binding to DNA and coordinated changes in chromatin structure. The primary protein components of chromatin are the his...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 10–16
Keywords: Acetylations, Amplification, Cancer, Cell culture, Chromatin immunoprecipitation, Cloning, DNA purification, Diseases, Epigenetics, Gene expression, Immunoprecipitation, Indicators, Methylations, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sequencing, Transcription, Whole genome amplification

Whole Gene Amplification from Archived Formalinfixed, Paraffin-embedded Tissues

Archived formalin-fixed and paraffin-embedded (FFPE) tissue collections represent invaluable resources for studying pathogenesis of cancer and a variety of other diseases. The possibility of retrospe...
Rosemarie Walter, James Eliason,* Yezhou Sun,* Joseph Cortese,* and Richard Everson*
Asterand, Inc., Detroit, MI, USA *Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI, USA
Keywords: Amplification, Cancer, Cell culture, Clinical, Diseases, Genetic, Genomics, Molecular probes, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Whole genome amplification

Whole Genome Amplification

The GenomePlex® WGA family of products are derived from the proprietary amplification method that is based upon random fragmentation of the genome into a series of overlapping, short templates. The r...
Keywords: Amplification, Cancer, Cell disruption, DNA purification, Electrophoresis, Gel electrophoresis, Genetic, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Whole genome amplification

Protocols

Animal Tissue DNA-Extraction & WGA Amplification Protocol

Animal tissue is a common source of material when performing genetic analysis. The protocol below is a simple method of extracting DNA from the animal sample. Once the DNA has been isolated, it can t...
Keywords: Absorption, Amplification, Cell disruption, Genetic, Molecular probes, Nucleic acid denaturation, Polymerase chain reaction, Purification, Whole genome amplification

Blood Card - Extraction & Amplification WGA Protocol

Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, which may hinder the researcher’s ability to perform downstream...
Keywords: Absorption, Amplification, Cell disruption, Molecular probes, Nucleic acid denaturation, Polymerase chain reaction, Purification, Whole genome amplification

Buccal DNA Extraction & WGA Amplification Protocol - Technical Documents

This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal cell swabs. Buccal swabs are a convenient method of acquiring a DNA sample. Once the DNA ...
Keywords: Absorption, Amplification, Cell disruption, Molecular probes, Nucleic acid denaturation, Polymerase chain reaction, Purification, Whole genome amplification

Chromatin Immunoprecipitation Kit CHP1 Protocol

Product Description Storage/Stability Workflow Preparation Instructions Procedure Analysis Troubleshooting Guide Frequently Asked Questions Materials References Poster & Application Note
Keywords: AGE, Amplification, Cell culture, Cell disruption, Centrifugation, Chromatin immunoprecipitation, DNA purification, DNA replication, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Genomics, Homogenization, Immunoprecipitation, Individual protein Immunoprecipitation, Molecular biology, Molecular probes, Nucleic acid annealing, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, Recombination, Sample preparations, Size-exclusion chromatography, Sonication, Transcription, Transduction, Whole genome amplification

Extraction & Amplification of whole blood using WGA-Protocol

Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This can hinder the researcher’s ability to perf...

FFPE Tissue

Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases. Since the archived DNA is usually available...
Keywords: Amplification, Cell disruption, DNA purification, Diseases, Evaporation, Gene expression, Molecular biology, Purification, Whole genome amplification

GenomePlex Whole Genome Amplification Kit Protocol

Product Description Components Storage/Stability Procedure Troubleshooting Guide Frequently Asked Questions Precautions and Disclaimer Materials References
Keywords: Absorption, Amplification, Centrifugation, Diagnostic, Dialysis, Genomics, Molecular biology, Molecular probes, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Whole genome amplification

GenomePlex® WGA Reamplification Kit Protocol (WGA3)

The GenomePlex WGA Reamplification Kit allows subsequent reamplifications of the WGA product with little genetic bias.  This kit contains an optimized enzyme that decreases the background in the reac...
Keywords: Amplification, Diagnostic, Dialysis, Genetic, Genomics, Molecular probes, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Whole genome amplification

Integration of Sigma® GenomePlex® WGA with the Affymetrix CGH Microarray Workflow

The GenomePlex® WGA amplification product is suitable as a microarray target for expression analysis on the Affymetrix platform, and can be readily integrated into existing Affymetrix workflows for C...
Keywords: Amplification, Centrifugation, Gene expression, Microarray Analysis, Nucleic acid hybridization, Polymerase chain reaction

Plant DNA extraction & WGA Amplification-protocol

Extracting DNA from plant tissue is a complicated process due to the tough cell wall that surrounds most plant cells. Genomic DNA from plant material can be damaged during the extraction process, res...
Keywords: Absorption, Amplification, Cell disruption, Centrifugation, Filtration, Molecular probes, Nucleic acid denaturation, Polymerase chain reaction, Precipitation, Purification, Whole genome amplification

Protocol for DNA extraction & WGA Amplification from Soil Samples

Genomic DNA from soil samples can be easily damaged by nucleases and contaminating debris, resulting in low DNA yields. As a result, the researcher’s ability to perform downstream analysis may be com...
Keywords: Absorption, Amplification, Molecular probes, Nucleic acid denaturation, Polymerase chain reaction, Purification, Whole genome amplification

Saliva DNA Extraction & WGA Amplification Protocol

Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit (NA2010), GenElute Mammalian Genomi...
Keywords: Absorption, Amplification, Molecular probes, Nucleic acid denaturation, Polymerase chain reaction, Purification, Whole genome amplification

Related Content

Automated Protocols for Life Science Research

Our scientists have validated all of the automated methods available below. Automation protocols are also provided as instruction for each of the methods. These protocols supply information such as i...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

Related Products

recommended

Product #

Description

Add to Cart

Z369659 SealPlate® film, non-sterile
NA1020 GenElute PCR Clean-Up Kit, sufficient for 70 purifications
W4502 Water, Nuclease-Free Water, for Molecular Biology

related product

Product #

Description

Add to Cart

WGA2 GenomePlex® Complete Whole Genome Amplification (WGA) Kit, Optimized kit with enzyme for amplifying a variety of DNA including FFPE tissue
WGA3 GenomePlex® WGA Reamplification Kit, Reamplification of WGA product with minimal bias
WGA4 GenomePlex® Single Cell Whole Genome Amplification Kit, Amplify genome of a single cell
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?