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WGA3 Sigma-Aldrich

GenomePlex® WGA Reamplification Kit

Reamplification of WGA product with minimal bias

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Description

Features and Benefits

• Starting material: 10 ng
• Expected yield: 5 - 10 μg
• Time required: ~1.5 hours
• Provides a method to accurately amplify whole genome amplified DNA
• Successive reamplification with WGA-3 provides DNA with little genetic bias when compared to the original genome
• Maintains representation of the entire genome through subsequent reamplifications
• Preserves precious source material by amplifying nanogram amounts of starting genomic DNA into microgram yields (on average up to 500-fold)
• Minimal allele drop out maintained through five successive cycles
• Increases the number of downstream assays and allows more information to be gathered on that sample
• Provides the opportunity to re-amplify the WGA DNA without having to purchase an entire WGA kit

General description

GenomePlex WGA Reamplification Kit utilizes a proprietary amplification method that is based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.

Other Notes

The sequences of the universal primers provided in this kit are considered proprietary.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

GenomePlex is a registered trademark of Takara Bio USA, Inc.

Application

GenomePlex® WGA Reamplification Kit has been used to generate DNA probes (μBm-probe and μBsm-probe) labeled with digoxigenin-11-dUTP.

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Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .2 mL SDS D7295

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WGA DNA Polymerase    

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Amplification of genome-representative DNA from limited sources with GenomePlex® WGA technology for use in genetic alterations studies

Modern technologies developed in recent years have prompted new and exciting approaches to support biomedical research. The ability to study the entire human genome using limited sources of genomic m...
Victoria Rusakova & Lukasz Nosek
Sigma-Aldrich Biotechnology
Keywords: Amplification, Clinical, DNA purification, Diagnostic, Diseases, Functional genomics, Genetic, Genetics, Genomics, In Situ hybridization, Microarray Analysis, Nucleic acid hybridization, Polymorphisms, Purification, Sequencing

Histone Modification and Chromatin Remodeling

Gene expression is governed by complex mechanisms including transcription factor binding to DNA and coordinated changes in chromatin structure. The primary protein components of chromatin are the his...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 10–16
Keywords: Acetylations, Amplification, Cancer, Cell culture, Chromatin immunoprecipitation, Cloning, DNA purification, Diseases, Gene expression, Immunoprecipitation, Indicators, Methylations, Microarray Analysis, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sequencing, Transcription, Whole genome amplification

Whole Genome Amplification

The GenomePlex® WGA family of products are derived from the proprietary amplification method that is based upon random fragmentation of the genome into a series of overlapping, short templates. The r...
Keywords: Amplification, Cancer, Cell disruption, DNA purification, Electrophoresis, Gel electrophoresis, Genetic, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Whole genome amplification

Protocols

Buccal DNA Extraction & WGA Amplification Protocol - Technical Documents

This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample. Once the DNA is is...
Keywords: Absorption, Amplification, Cell disruption, Molecular probes, Nucleic acid denaturation, Polymerase chain reaction, Purification, Whole genome amplification

Chromatin Immunoprecipitation Kit CHP1 Protocol

Product Description Storage/Stability Workflow Preparation Instructions Procedure Analysis Troubleshooting Guide Frequently Asked Questions Materials References Poster & Application Note
Keywords: AGE, Amplification, Cell culture, Cell disruption, Centrifugation, Chromatin immunoprecipitation, DNA purification, DNA replication, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Genomics, Homogenization, Immunoprecipitation, Individual protein Immunoprecipitation, Molecular biology, Molecular probes, Nucleic acid annealing, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, Recombination, Sample preparations, Size-exclusion chromatography, Sonication, Transcription, Transduction, Whole genome amplification

GenomePlex Whole Genome Amplification Kit Protocol

Product Description Components Storage/Stability Procedure Troubleshooting Guide Frequently Asked Questions Precautions and Disclaimer Materials References
Keywords: Absorption, Amplification, Centrifugation, Diagnostic, Dialysis, Genomics, Molecular biology, Molecular probes, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Whole genome amplification

GenomePlex® WGA Reamplification Kit Protocol (WGA3)

The GenomePlex WGA Reamplification Kit allows subsequent reamplifications of the WGA product with little genetic bias.  This kit contains an optimized enzyme that decreases the background in the reac...
Keywords: Amplification, Diagnostic, Dialysis, Genetic, Genomics, Molecular probes, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Whole genome amplification

Plant DNA extraction & WGA Amplification-protocol

Extracting DNA from plant tissue is a complicated process due to the tough cell wall that surrounds most plant cells. Genomic DNA from plant material can be damaged during the extraction process, res...

WGA4 - GenomePlex® Single Cell Whole Genome Amplification Kit

Product Description Reagents Provided Storage/Stability Procedure References Troubleshooting Guide FAQs Posters and Publications Downstream applications         Gel Electrophoresis & qPCR         CGH...
Keywords: Amplification, Cell disruption, Centrifugation, Degradations, Diagnostic, Electrophoresis, Gel electrophoresis, Genomics, Microarray Analysis, Molecular biology, Molecular probes, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Whole genome amplification

Related Content

GenomePlex® Amplification of ChIP DNA

ChIP-chip is a powerful tool that combines chromatin immunoprecipitation (ChIP) with microarrays to study protein-DNA interactions genome-wide. The ChIP step utilizes an antibody directed against a t...
Keywords: Acetylations, Amplification, Cancer, Cell culture, Chromatin immunoprecipitation, Gene expression, Immunoprecipitation, Methylations, Microarray Analysis, Nucleic acid hybridization, Sample preparations, Transcription, Whole genome amplification, transformation

PCR Selection Guide

We offer a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from routine to enh...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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