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WGA4 Sigma-Aldrich

GenomePlex® Single Cell Whole Genome Amplification Kit

Amplify genome of a single cell

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Description

General description

GenomePlex Single Cell Whole Genome Amplification Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.

Application

GenomePlex® Single Cell Whole Genome Amplification Kit has been used:
• to amplify the isolated DNA
• in whole genome amplification (WGA)
• to amplify the microdissected DNAs

Features and Benefits

• Starting material: 1 cell
• Expected yield: 5 - 10 μg
• Time required: ~4 hours
• Abundant DNA yield within four hours
• Complete representation of the entire genome with minimal allele bias
• Includes all of the reagents necessary for cell lysis and subsequent whole genome amplification
• Designed to amplify the genome of a single cell from any source including lymphocytes, cancer cells, epithelial cells, fibroblast, amniotic cells, polycarbonate fixed cells, and plant cells

Other Notes

The sequences of the universal primers provided in this kit are considered proprietary.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

GenomePlex is a registered trademark of Takara Bio USA, Inc.

Kit component also available separately

Description

Product #

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10x Single Cell Lysis & Fragmentation Buffer SDS L1043
Proteinase K from Tritirachium album, buffered aqueous glycerol solution, for molecular biology, ≥800 units/mL SDS P4850
Water, Nuclease-Free Water, for Molecular Biology SDS W4502
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Can the amplified Omniplex products resulting from the use of Product WGA4, GenomePlex® Single Cell Whole Genome Amplification Kit, be used for TA cloning?
Yes. Please ensure that a final extension of 7-30 minutes at 72°C is included after amplification if performing subsequent TA cloning reactions.
CanProduct WGA4, GenomePlex® Single Cell Whole Genome Amplification Kit, be used with purified DNA?
Yes. The kit is designed to amplify the small amount of DNA that is present in a single cell (3-5 pg). It will also work with small amounts of purified DNA; we recommend less than 100 pg.
What kinds of cells can be used as starting materials with Product WGA4, GenomePlex® Single Cell Whole Genome Amplification Kit?
The cell lysis method involves treatment with proteinase K and heat. Most cells will be lysed under these conditions.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles

Articles

Amplification of genome-representative DNA from limited sources with GenomePlex® WGA technology for use in genetic alterations studies

Modern technologies developed in recent years have prompted new and exciting approaches to support biomedical research. The ability to study the entire human genome using limited sources of genomic m...
Victoria Rusakova & Lukasz Nosek
Sigma-Aldrich Biotechnology
Keywords: Amplification, Clinical, DNA purification, Diagnostic, Diseases, Functional genomics, Genetic, Genetics, Genomics, In Situ hybridization, Microarray Analysis, Nucleic acid hybridization, Polymorphisms, Purification, Sequencing

Histone Modification and Chromatin Remodeling

Gene expression is governed by complex mechanisms including transcription factor binding to DNA and coordinated changes in chromatin structure. The primary protein components of chromatin are the his...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 10–16
Keywords: Acetylations, Amplification, Cancer, Cell culture, Chromatin immunoprecipitation, Cloning, DNA purification, Diseases, Epigenetics, Gene expression, Immunoprecipitation, Indicators, Methylations, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sequencing, Transcription, Whole genome amplification

Whole Genome Amplification for Single Cell Biology - Technical Article

The ultimate biological unit lies within a single cell. Many biological disciplines have taken aim to elucidate the causes of cellular differentiation at this level. The secret triggers that signal h...
Ernie Mueller and Chad Brueck
St. Louis, MO, USA
Keywords: Amplification, Cancer, Cell biology, Cell disruption, Diseases, Drug discovery, Epigenetics, Gene expression, Genetic, Genomics, Mass selective detector, Metabolites, Metabolomics, Microarray Analysis, Microscopy, Molecular biology, Nucleic acid annealing, Nucleic acid hybridization, Peptide synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Whole genome amplification

Whole Genome Amplification

The GenomePlex® WGA family of products are derived from the proprietary amplification method that is based upon random fragmentation of the genome into a series of overlapping, short templates. The r...
Keywords: Amplification, Cancer, Cell disruption, DNA purification, Electrophoresis, Gel electrophoresis, Genetic, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Whole genome amplification

Protocols

Buccal DNA Extraction & WGA Amplification Protocol - Technical Documents

This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample. Once the DNA is is...
Keywords: Absorption, Amplification, Cell disruption, Molecular probes, Nucleic acid denaturation, Polymerase chain reaction, Purification, Whole genome amplification

Integration of Sigma® GenomePlex® WGA with the Affymetrix CGH Microarray Workflow

The GenomePlex® WGA amplification product is suitable as a microarray target for expression analysis on the Affymetrix platform, and can be readily integrated into existing Affymetrix workflows for C...
Keywords: Amplification, Centrifugation, Gene expression, Microarray Analysis, Nucleic acid hybridization, Polymerase chain reaction

Plasma Serum DNA extraction & WGA Amplification Protocol

Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples. One must use a robust DNA purification scheme capable of ...
Keywords: Absorption, Amplification, Cell disruption, Centrifugation, DNA purification, Molecular biology, Molecular probes, Nucleic acid denaturation, Polymerase chain reaction, Purification, Whole genome amplification

WGA4 - GenomePlex® Single Cell Whole Genome Amplification Kit

Product Description Reagents Provided Storage/Stability Procedure References Troubleshooting Guide FAQs Posters and Publications Downstream applications         Gel Electrophoresis & qPCR         CGH...
Keywords: Amplification, Cell disruption, Centrifugation, Degradations, Diagnostic, Electrophoresis, Gel electrophoresis, Genomics, Microarray Analysis, Molecular biology, Molecular probes, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Whole genome amplification

Related Content

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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