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WHAWB120205 Sigma

Whatman® FTA® card technology

FTA classic card with 4 sample areas per card, 125 μL maximum volume/sample area, 500 μL maximum total volume/card, pkg of 100 ea

Synonym: Z719730, whatman fta cards

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Properties

Related Categories Labware, Manufacturer Browser, Whatman, Whatman FTA Card Technology, Whatman FTA Cards More...
packaging   pkg of 100 ea
mfr. no.   Whatman Article No., 28421884 (US reference)
  Whatman, WB120205
suitability   suitable for (isolation, purification and storage of nucleic acids)

Description

Application

Use FTA card technology for a wide range of applications:
• Forensics
• Transgenic identification
• Transfusion medicine
• Plasmid screening
• Food and agriculture testing
• Drug discovery
• Genomics
• STR analysis
• Animal identification
• Whole genome amplification
• Molecular biology

Features and Benefits

• Capture nucleic acid in one easy step
• Captured nucleic acid is ready for downstream applications in less than 30 minutes
• DNA collected on FTA Cards is preserved for years at room temperature
• FTA Cards are stored at room temperature before and after sample application, reducing the need for laboratory freezers
• Suitable for virtually any cell type
• Indicating FTA Cards change color upon sample application to facilitate handling of colorless samples
• FTA Cards are available in a variety of configurations to meet application requirements
• Custom configurations are available on request

General description

FTA Classic Card
Four sample areas for application of up to 500 μl whole blood or 100 μl plant homogenate per card. Convenient for multiple applications of the same specimen or collection of multiple animal or plant samples on one card. Different samples can be processed independently.

FTA cards utilize Whatman FTA technology that simplifies the handling and processing of nucleic acids. FTA Cards contain chemicals that lyse cells, denature proteins and protect nucleic acids from nucleases, oxidation and UV damage. FTA cards rapidly inactivate organisms, including blood-borne pathogens, and prevent the growth of bacteria and other microorganisms.

Capture nucleic acids in one easy step
Simply apply your sample to the FTA Card. Cell membranes and organelles are lysed and the released nucleic acids are entrapped in the fibers of the matrix. The nucleic acids remain immobilized and are preserved for transport, immediate processing or long-term room temperature storage.

Since captured nucleic acids are preserved, FTA Cards facilitate sample collection in remote locations and simplify sample transport. For example, you can collect samples in the field without worrying about immediate refrigeration. Ship your samples back to the laboratory without expensive special handling or dry ice and process at your convenience.
Indicating FTA Cards are recommended for colorless samples. These FTA Cards change from pink to white when sample is applied, verifying the location of the sample.

FTA Cards can be used with virtually any sample type:
• Blood
• Cultured cells
• Buccal cells
• Plasmids
• Solid tissue

Captured nucleic acid is ready for downstream applications in less than 30 minutes
Captured nucleic acids are ready for purification when you are. Just take a punch from the FTA Card, wash with FTA Purification Reagent and rinse with TE-1 (10 mM Tris-HCI, 0.1 mM EDTA, pH 8) buffer. DNA on the washed punch is ready to use in applications such as PCR, SNP analysis, and real-time PCR. Since PCR products remain in solution, the punch can be used for multiple amplifications.

Store nucleic acids at room temperature for years

Genomic DNA stored on FTA Cards at room temperature for over 17 years (and counting) has been successfully amplified by PCR. RNA, being chemically less stable than DNA, is best analyzed upon return of samples to the laboratory. Frozen storage is helpful for RNA preservation.

Sample integrity is optimized when FTA Cards are stored in a Multi-Barrier Pouch with a Desiccant Packet.

FTA Cards offer a compact room-temperature storage system that reduces the need for precious freezer space.

Other Notes

Field of Use : The processing of genetic material whereby a solid matrix is utilized for the isolation, storage, purification or processing.

Legal Information

FTA is a registered trademark of GE Healthcare

Whatman is a registered trademark of GE Healthcare

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis


Same Products - New Packaging
Protocols & Articles

Articles

Instructions for EasiCollect™

EasiCollect is an all-in-one device for the collection and storage of cheek cells. Cells are captured on the foam applicator by swabbing the inside of both cheeks and then transferred to an Indicatin...
Keywords: Amplification, Diagnostic, Environmental, Polymerase chain reaction, Purification, Sample preparations

RNA isolation from blood, cultured cells, and animal or plant tissue on Whatman™ FTA™ cards

Whatman FTA cards are used for biological sample collection, storage, transport, and isolation of high-quality DNA for downstream applications. In the following studies, FTA cards were evaluated for ...
Keywords: AGE, Amplification, Cell culture, Cell disruption, Centrifugation, Degradations, Diseases, Gene expression, Genomics, Homogenization, Immobilization, Metabolism, Phase transitions, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA purification, Transcription

Reliable extraction of DNA from Whatman™ FTA™ cards

This study examined the yield and quality of DNA from samples applied to Whatman FTA cards, using five common methods of DNA extraction. The data demonstrate that all five methods yield purified DNA ...
Keywords: Amplification, Cell disruption, Degradations, Genetic, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Separation, Size-exclusion chromatography

Protocols

Comparison of Treated Versus Untreated Matrices for DNA stabilization

Extracted from Nucleic Acid Sample Preparation for Downstream Analyses, GE Healthcare, 2007
Keywords: Polymerase chain reaction, Sample preparations

Genomic DNA Purification from Sample Applied to FTA Cards

Extracted from Nucleic Acid Sample Preparation for Downstream Analyses, GE Healthcare, 2007
Keywords: Polymerase chain reaction, Purification, Sample preparations

Nucleic Acid Sample Preparation Methodology

Spectrophotometry can be used to estimate DNA or RNA concentration and to analyze the purity of the preparation. Typical wavelengths for measurement are 260 nm and 280 nm. In addition, measurements a...
Keywords: AGE, Absorption, Amplification, Buffers, Carcinogens, Centrifugation, Cloning, Degradations, Digestions, Electrophoresis, Extinction coefficient, Gel electrophoresis, Microarray Analysis, Microscopy, Mutagens, Nucleic acid purification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Pulsed field gel electrophoresis, Purification, Reductions, Sequencing, Teratogens, Transfection

Options for Sample Collection, Transport, Archiving, and DNA Purification

Extracted from Nucleic Acid Sample Preparation for Downstream Analyses, GE Healthcare, 2007
Keywords: Amplification, DNA purification, Phase transitions, Polymerase chain reaction, Purification, Sample preparations

Troubleshooting Common Problems that can occur during Genomic DNA Preparation

Some of the more common problems that can occur during genomic DNA preparation are described in Table 4.4. Refer to the instructions provided with your genomic DNA preparation product for more detail...
Keywords: Amplification, Degradations, Homogenization, Precipitation, Purification, Solvents

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