• USA Home
  • WTA1 - TransPlex® Whole Transcriptome Amplification Kit

EMAIL THIS PAGE TO A FRIEND
WTA1 Sigma-Aldrich

TransPlex® Whole Transcriptome Amplification Kit

DNA polymerase separate.

  •  NACRES NA.55

Purchase

Description

General description

TransPlex, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3′-bias. Microgram quantities of amplification product generated from tissue, cultured cells, formalin-fixed samples, or serum are suitable for downstream applications such as qPCR and microarray analyses.

Application

TransPlex®  Whole Transcriptome Amplification Kit has been used to synthesize double-stranded cDNA.

Suitable for use with downstream applications including:
• qPCR
• microarray analysis
• cloning

Features and Benefits

• Amplification of total RNA in less than 4 hours with less than 30 minutes of "hands-on" time
• Only 5 ng of starting material required to produce a highly representative library from total RNA
• Microgram quantities of amplification product generated from intact RNA from tissue, cultured cells, serum, or degraded RNA from formalin-fixed paraffin-embedded samples

Principle

The WTA process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. As polymerization proceeds, displaced single strands serve as new templates for primer annealing and extension. The resultant OmniPlex cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence, is then amplified by PCR with the universal primer to produce WTA product.

Legal Information

TransPlex is a registered trademark of Rubicon Genomics, Inc.

Kit component also available separately

Description

Product #

Add to Cart

Water, Nuclease-Free Water, for Molecular Biology SDS W4502
Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent SDS D7295

Kit component only

Description

Product #

Add to Cart

WTA Library Synthesis Buffer    
WTA Library Stabilization Solution    
WTA Library Synthesis Enzyme    
WTA Amplification Master Mix    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the concentration of the dNTPs supplied with the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1?
The dNTP Mix (D6193) is supplied as 10 mM each.
Is the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, also applicable for ribosomal RNA and/or small RNA amplification?
Using the kit as is, Ribosomal RNA does appear to amplify. However, we have not quantitated how well it is amplified.
What is the difference between WTA and WGA? I use the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1.
The starting material for WTA is RNA, whereas for WGA it is genomic DNA.After the RNA is reverse-transcribed into the DNA complement by WTA, the OmniPlex library is formed. This library is in turn amplified by PCR.
CanTransPlex® Whole Transcriptome Amplification Kit, Product WTA1, be used to amplify archival fixed tissue or degraded samples?
Yes, the WTA kit effectively amplifies degraded RNA, including formalin-fixed and paraffin-embedded samples.
Can TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, be used for downstream applications?
Upon creation of a cDNA library, quantitative qPCR or microarray analysis may be performed
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions
Protocols & Articles

Articles

Histone Modification and Chromatin Remodeling

Gene expression is governed by complex mechanisms including transcription factor binding to DNA and coordinated changes in chromatin structure. The primary protein components of chromatin are the his...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 10–16
Keywords: Acetylations, Amplification, Cancer, Cell culture, Chromatin immunoprecipitation, Cloning, DNA purification, Diseases, Epigenetics, Gene expression, Immunoprecipitation, Indicators, Methylations, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sequencing, Transcription, Whole genome amplification

Whole Transcriptome Amplification FAQs

How does WTA work? What type of RNA may be used? How much RNA is required to successfully perform WTA amplification? Can WTA2 be used to amplify archival fixed tissue or degraded samples? How can I o...
Keywords: Amplification, Buffers, Cloning, DNA purification, Microarray Analysis, Nucleic acid annealing, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification

Protocols

Integration of Sigma® TransPlex® WTA and Complete WTA2 Kits with the Agilent Microarray Workflow

Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be readily integrated into existing Agilent workflows...
Keywords: Amplification, Centrifugation, Gene expression, Microarray Analysis, Nucleic acid hybridization, Polymerase chain reaction

Integration of Sigma® TransPlex® WTA and the Complete WTA2 Kits with the Affymetrix Microarray Workflow

Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be incorporated into existing Affymetrix workflows th...
Keywords: Amplification, Centrifugation, Functional genomics, Gene expression, Genomics, Microarray Analysis, Nucleic acid hybridization, Polymerase chain reaction, Purification

Integration of Sigma® TransPlex® WTA and the Complete WTA2 Kits with the Illumina Microarray Workflow

Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be incorporated into existing Illumina workflows.
Keywords: Amplification, Digestions, Gene expression, Microarray Analysis, Molecular probes, Nucleic acid hybridization, Polymerase chain reaction

Integration of Sigma® TransPlex® WTA and the Complete WTA2 Kits with the NimbleGen™ Expression Microarray Workflow

Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be readily integrated into existing NimbleGen workflo...
Keywords: Amplification, Centrifugation, Gene expression, Microarray Analysis, Polymerase chain reaction

TransPlex® Whole Transcriptome Amplification Protocol

TransPlex, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3'-bias. Microgram quantities of ...
Keywords: Amplification, Centrifugation, Cloning, Condensations, DNA purification, Gene expression, Genomics, Microarray Analysis, Molecular biology, Molecular probes, Nucleic acid annealing, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Purification, Sequencing

Related Content

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

WTA2 Complete Whole Transcriptome Amplification Kit, DNA polymerase included, Complete Kit with optimized enzyme to amplify total RNA in <4 hours, no 3′ bias
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?