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Y0626 Sigma-Aldrich

Yeast Nitrogen Base Without Amino Acids

Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements

  •  NACRES NA.85

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Description

General description

Yeast Nitrogen Base is considered ideal for culturing wild type yeast. The presence of amino acid is known to prevent selectable marker utilization. Hence, this media is formulated without amino acids and is useful for cloning and manipulation of yeast artificial chromosome.

Application

Yeast Nitrogen Base Without Amino Acids has been used:
• in synthetic complete supplement mixture (SC)-Leu media used for Y. lipolytica culturing and characterization
• as a component of selective liquid media for uracil auxothrophic growth in yeast
• to evaluate the transformation efficiencies of S. cerevisiae strains, under different nutrient conditions

Components

Component, amount (μg/L unless indicated)

Nitrogen Sources:
Ammonium sulfate, 5.0 g/L

Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400

Trace Elements:
Boric acid, 500
Copper sulfate, 40
Potassium iodide, 100
Ferric chloride, 200
Manganese sulfate, 400
Sodium molybdate, 200
Zinc sulfate, 400

Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L

Reconstitution

Make a 10x concentrated stock solution by stirring to suspend 6.8g yeast nitrogen base powder , 5 g glucose, and 5-10 mg of appropriate amino acid in 100 mL water. Warm if necessary to aid solubilization. Filter sterilize and store at 2-8°C.

Dilute the 10x concentrated stock to a 1x working solution by adding 100mL concentrated stock to 900mL sterile water.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Culture Media and Tests for Beverage Testing

Spoilage organism testing is a crucial step within the beverage industry to enable the safe release of drinks to the market. Requirements can vary from industry to industry, depending on different sp...
Keywords: Culture media, Fermentation, Magnetic resonance spectroscopy

Culture Method for Quality Control of Beer

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media. One of the first steps is the filtration of the beer followed by an incubation of the filt...
Jvo Siegrist
Microbiology Focus Edition 1.4
Keywords: Fermentation, Filtration, Magnetic resonance spectroscopy, Metabolism, Microbiology

Introduction to Microbial Media

Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation

Introduction to Yeast Media

Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced. They are relatively easy to grow under laboratory conditions. Moreover, despite thei...
Keywords: Culture media, Fermentation, Gene expression, Indicators, Molecular biology, Sterilizations, Transcription, Vitamins, transformation

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. We are an essential part of your...
Keywords: Cell disruption, Gene expression, transformation

Yeast Drop Out Bulletin

Catalog Number Y1501: without uracil Catalog Number Y1751: without histidine Catalog Number Y1896: without lysine Catalog Number Y1376: without leucine Catalog Number Y1876: without tryptophan Catalo...
Keywords: Filtration, transformation

Protocols

Yeast Growth Protocols | Growth and Maintenance of Yeast

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Moreover, replica plating and mutant isolation of yeast cells can be done with relativ...
Keywords: Culture media, Detergents, Genetic, transformation

Peer-Reviewed Papers
15

References

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