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Y0875 Sigma-Aldrich

Select Yeast Extract

for use in microbial growth medium

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Properties

Related Categories Extracts, Media Components, Microbial Media for Molecular Biology and Protein Expression, Molecular Biology
Quality Level   200
grade   for molecular biology
sterility   non-sterile
form   powder
pH range   6.5 - 7.5 (10% solution)
solubility   H2O: 100 mg/mL, clear to slightly hazy, yellow to brownish-yellow
storage temp.   room temp
suitability   nonselective for Escherichia coli
  nonselective for coliforms

Description

General description

Yeast extract is the water-soluble portion of autolyzed yeast, often used in culture media. Yeast extract contains a mixture of amino acids, peptides, water soluble vitamins and carbohydrates.
Yeast extract is used in all solid media, on which stock cultures are maintained, due to its ability in promoting growth of most bacterial contaminants, thereby making them visible to identify. Yeast extract does not enhance the growth of wild-type cells but essential for auxotrophic mutants.

Application

Suitable for use as nutritional source in microbial media.
Select Yeast Extract has been used as a supplement in the minimal sporulating medium (MSL) for the secretory production of human tumor necrosis factor-α (TNFα) using the carboxypeptidase Y (Cpy) signal peptide. It has also been used in the isolation of halophilic archaea.

Yeast extract is used as a bacteriological media component for a variety of microorganisms and molecular genetics applications.

Other Notes

Water soluble portion of autolyzed yeast with intact B-complex vitamins. Yeast extract is a mixture of amino acids, peptides, water soluble vitamins and carbohydrates and can be used as additive for culture media.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Introduction to Microbial Media

Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. We are an essential part of your...
Keywords: Cell disruption, Gene expression, transformation

Protocols

Microbial Growth Protocols

From our library of Protocols, Sigma-Aldrich presents Microbial Growth Protocols
Keywords: Antibiotics

Peer-Reviewed Papers
15

References

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