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Y1250 Sigma-Aldrich

Yeast Nitrogen Base With Amino Acids

Non-selective yeast growth medium

  •  NACRES NA.85



Related Categories Cloning and Expression, Core Bioreagents, HIS Select Supporting Products and Reagents, HIS-Select, Microbial Media,
Quality Level   200
grade   for molecular biology
sterility   non-sterile
form   powder
pH range   5.3 - 5.5 (0.67% solution)
storage temp.   room temp
suitability   nonselective for Candida spp.
  nonselective for Pichia spp.
  nonselective for Saccharomyces spp.
  nonselective for Zygosaccharomyces spp.


General description

Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains amino acids, nitrogen, vitamins, trace elements and salts.


Yeast Nitrogen Base With Amino Acids has been used:
• in the ZYP-31 media preparation for E.coli
• in synthetic dextrose (SD) medium to evaluate the influence of nitrogen source
• to culture Candida utilis for the production of 13C-labeled yeast extract as internal standard
• in the preparation of imaging medium


250 g in poly bottle


Component, amount (μg/L unless indicated)

Nitrogen Sources:
Ammonium sulfate, 5.0 g/L

Amino Acids:
L-histidine, 10 mg/L
DL-methionine, 20 mg/L
DL-tryptophan, 20 mg/L

Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400

Trace Elements:
Boric acid
Copper sulfate
Potassium iodide
Ferric chloride
Magnesium sulfate
Sodium molybdate
Zinc sulfate

Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L


Make a 10x concentrated stock solution by stirring to suspend 6.7g yeast nitrogen base powder and 5 g glucose in 100 mL water. Warm if necessary to aid solubilization. Filter sterilize and store at 2-8°C.

Dilute the 10x concentrated stock to a 1x working solution by adding 100mL concentrated stock to 900mL sterile water.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles


Introduction to Microbial Media

Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation

Introduction to Yeast Media

Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced. They are relatively easy to grow under laboratory conditions. Moreover, despite thei...
Keywords: Culture media, Fermentation, Gene expression, Indicators, Molecular biology, Sterilizations, Transcription, Vitamins, transformation

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. We are an essential part of your...
Keywords: Cell disruption, Gene expression, transformation


Yeast Growth Protocols | Growth and Maintenance of Yeast

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Moreover, replica plating and mutant isolation of yeast cells can be done with relativ...
Keywords: Culture media, Detergents, Genetic, transformation

Peer-Reviewed Papers


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Y0626 Yeast Nitrogen Base Without Amino Acids, Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements

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