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Y1253 Sigma-Aldrich

Anti-Y14 antibody, Mouse monoclonal

clone 4C4, purified from hybridoma cell culture

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics and Nuclear Signaling, Antibodies for Gene Regulation,
conjugate   unconjugated
clone   4C4, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 0.5-1 μg/mL using nuclear extract of HeLa cells
species reactivity   Xenopus, human
mol wt   antigen ~24 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2b
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   Q9Y5S9
Gene Information   Xenopus laevis ... Rbm8(399167)
human ... RBM8A(9939)

Description

General description

RNA binding motif protein 8A (RBM8A) or Y14 is part of the RNA binding motif family. It has a molecular weight of 26kDa. The protein is localized to the nucleus and shuttles between the nucleus and cytoplasm.

Immunogen

full length of human Y14.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

RNA binding motif protein 8A (RBM8A) or Y14 acts as a modulator of apoptosis. It has a role in the formation of exon-junction binding complex. The protein is the core factor of nonsense-mediated mRNA decay and is involved in NMD-mediated mRNA surveillance. RBM8A is expressed in various tumors and cancers. The protein binds to the mRNA cap and inhibits the function of the enzyme, decapping mRNA 2 (DCP2).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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