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Y1625 Sigma

Yeast Extract

for use in microbial growth medium

Synonym: Select Yeast Extract

Purchase

Properties

Related Categories Broth / Media, Core Bioreagents, Extracts, Life Science Reagents for Cloning, Media Components,
Quality Level   PREMIUM
grade   for molecular biology
sterility   non-sterile
form   powder
Featured Industry   Agriculture
storage temp.   room temp
suitability   nonselective for Escherichia coli and Coliforms

Description

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

Other Notes

Water soluble portion of autolyzed yeast with intact B-complex vitamins. Yeast extract is a mixture of amino acids, peptides, water soluble vitamins and carbohydrates and can be used as additive for culture media.

Packaging

1 kg in poly bottle

250 g in poly bottle

Application

Suitable for use as nutritional source in microbial media.

Yeast extract is used as a bacteriological media component for a variety of microorganisms and molecular genetics applications.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
ZF6610000

Documents

Certificate of Analysis

Certificate of Origin


LC-MS Grade Solvents and Reagents

The Emprove® Program
Protocols & Articles

Articles

Introduction to Microbial Media

Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Culture media, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation

Protocols

A general protocol for the generation of Nanobodies for structural biology

Els Pardon a, b , Toon Laeremans a, b , Sarah Triest a, b , Søren G F Rasmussen c , Alexandre Wohlkönig a, b , Armin Ruf d , Serge Muyldermans b, e , Wim G J Hol f , Brian K Kobilka g , Jan Steyaert ...
Keywords: AGE, Adsorption, Amplification, Antibiotics, Biochemistry, Biotinylations, Buffers, Cancer, Centrifugation, Chromatography, Cloning, Coagulation, Crystallization, DNA purification, Deaminations, Degradations, Detergents, Digestions, Diseases, Drug discovery, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Genetic, Genomics, Hematology, High performance liquid chromatography, Immobilization, Immunology, Infectious Diseases, Neuroscience, Nucleic acid annealing, Nucleic acid hybridization, Peptide synthesis, Phase transitions, Polymerase chain reaction, Purification, Sample preparations, Separation, Sequencing, Size-exclusion chromatography, Sterilizations, Transfection, Western blot, transformation

High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

b Department of Pathology, Environmental Health and Radiation Oncology, UCLA School of Public Health and David Geffen School of Medicine
Keywords: Antibiotics, Biochemistry, Cell biology, Cell culture, Centrifugation, Environmental, Enzymology, Evaporation, Gene expression, Genetic, Genetics, Ion-exchange chromatography, Oncology, Sterilizations, transformation

Restriction Enzyme Cloning Manual Buffer Recipes

Tip 1: DNA is quite stable in TE buffer at 4ºC. If stored in elution buffer or water then freezing at -20 ºC is advised.
Keywords: Phase transitions, Sequencing, Sterilizations

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

Related Content

Plant Tissue Culture Products | Agriculture

Our combination of quality products, services and global business capabilities help you to maintain reagent consistency while ensuring suitability. Sigma-Aldrich allows you to standardize reagents ac...
Keywords: Antibiotics, Cell culture, Culture media, Gas chromatography, High performance liquid chromatography, Mass spectrometry, Vitamins, transformation

Peer-Reviewed Papers
15

References

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Y2627 2xYT Medium, EZMix Powder microbial growth medium
Y0875 Select Yeast Extract, for use in microbial growth medium

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