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EnPresso® Y Defined

Growth system for expressing protein in yeast



Related Categories Cell Biology, EnPresso Growth Systems for microbial culture, Life Science Research, Microbial Media for Molecular Biology and Protein Expression, Molecular Biology,
sterility   sterile; γ-irradiated
form   tablet
pH   6.8
shipped in   ambient
storage temp.   room temp


Biochem/physiol Actions

EnPresso® Y Defined is a pre-sterilized growth system designed to increase the yield of functional proteins expressed in Pichia pastoris when using AOX promoters. The system provides stress-free conditions, faster growth and eliminates the need for time-consuming media changes required when using conventional methanol-induction. EnPresso® growth systems provide optimal conditions for growth, metabolism and protein expression in microbial cultures. Protein yields are increased by enabling cultures to reach far higher cell densities than those achieved using conventional media.

By controlling growth rate and metabolism, a greater proportion of expressed protein can be correctly folded to improve solubility, minimize the risk of inclusion body formation, and ensure functionality of the final product.

EnPresso® growth systems maintain pH, provide adequate minerals, vitamins and trace elements to support growth, and use proprietary EnBase technology to ensure a constant, slow release of glucose from a polysaccharide substrate.

See all available products from EnPresso B Growth Systems.

Physical form

EnPresso® B is supplied in a kit providing sufficient reagents for 40 separate 50 ml cultures. Included in the kit:
120 tablets in 40 orange bags
1 bottle (20 ml) Reagent A

Legal Information

EnBase is a trademark of BioSilta Oy

EnPresso is a registered trademark of BioSilta Oy

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport


Certificate of Analysis

Milli-Q® Water Purification Solutions
Protocols & Articles


EnBase Technology - how it works (with webinar)

EnBase technology is a key element within every EnPresso® growth system, offering a novel approach to control the feeding, and thereby growth, of microbial cultures. A glucose-releasing agent breaks ...
Keywords: Deaminations, Metabolism, Metabolites, Respiration

EnPresso FAQs

A. EnPresso B growth system ready for use. Note small crystals of magnesium salts are visible – these do not affect performance.
Keywords: Anaerobic, Antibiotics, Cell disruption, Degradations, Gene expression, Indicators, Metabolism, Nutrition, Protein extraction, Purification, Reductions, Respiratory, Tagged proteins

EnPresso® Growth Systems: Revolutionizing the Role of Microbial Cell Culture

Growing cells to express proteins may seem just a small step on the way to achieving your research goals, but…
Keywords: Cell culture, Metabolites

EnPresso® Y Defined Growth (yeast protein data)

The first tablet-based, chemically-defined growth system for yeast, EnPresso Y Defined, enables production of more protein in less time from cultures of Pichia pastoris using AOX promoters with metha...

Introduction to Yeast Media

Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced. They are relatively easy to grow under laboratory conditions. Moreover, despite thei...
Keywords: Culture media, Fermentation, Gene expression, Indicators, Molecular biology, Sterilizations, Transcription, Vitamins, transformation

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have e...
Keywords: Amplification, Antibiotics, Cell disruption, Cloning, Culture media, Gene expression, Genetic, Genetics, Glycosylations, Molecular biology, Purification, Pyrogens, Transfection, transformation

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. We are an essential part of your...
Keywords: Cell disruption, Gene expression, transformation


EnPresso® Y Defined

EnPresso Y Defined is a pre-sterilized growth system designed to increase the yield of functional proteins expressed in Pichia pastoris when using AOX promoters. The system provides stress-free condi...
Keywords: Diagnostic, Filtration, Gene expression, Metabolism, Vitamins

Related Content

Synthetic Biology Resources

We are using our stregnths to help build the field of Synthetic Biology. Below is a collection of links to our favorite resources. In addtion to these resources, we also offer thousands of products f...
Keywords: Cell culture, Cloning, Gene expression, Genetic, Genetics, Metabolic Pathways, Metabolites, Molecular biology, Molecular biology techniques, Photosynthesis, Poisons, Polymerase chain reaction - quantitative, Transcription, Transfection

Webinar: High Protein Yields in Shaken Cultures by EnPresso® Growth System

EnPresso, a proprietary growth system from BioSilta Ltd, applies slow enzymatic glucose release to control the growth and protein synthesis rate in bacteria and yeasts. This high-cell-density growth ...
Keywords: Gene expression, Molecular biology

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